沉淀敲出法快速发现霍山产铁皮石斛生物碱及其组分抗氧化活性在线测定.docxVIP

文章编号: 1001-6880( 2014) 7-1000-05沉淀敲出法快速发现霍山产铁皮石斛生物碱及其组分抗氧化活性在线测定陈存武1，2 ，陈乃东1，2，3* ，孟云飞1 ，张艳1 ，胡建11 皖西学院生物与制药工程学院; 2 皖西中药与天然药物工程技术研究中心，六安 237012;3 安徽医科大学临床药理研究所，合肥 230032摘 要: 为了给霍山产铁皮石斛药效物质基础研究及抗氧化活性物质快速发现提供依据，在预实验基础上，采用鲁米诺-双氧水发光体系作为羟自由基清除活性评价平台，HPLC-UV-CL 联用在线测定了铁皮石斛生物碱部 位各组分清除自由基活性。以磷钼酸、碘-碘化钾为沉淀试剂敲出总生物碱提取物中生物碱组分，对比敲出前后 HPLC 图谱变化，确定铁皮石斛的生物碱组分。结果表明，在本实验条件下，霍山产铁皮石斛中检出的 5 种生物 碱中有 4 种可降低鲁米诺-双氧水发光体系的发光值。HPLC-UV-CL 可用于铁皮石斛生物碱清除自由基活性快 速测定。实验结果可为铁皮石斛质量控制、抗氧化活性成分定向分离的研究提供依据。关键词: 铁皮石斛; 沉淀敲出; 高效液相色谱-化学发光联用法; 生物碱中图分类号: Ｒ284. 1文献标识码: AQuick Ｒecognition of Unknown Alkaloids from Dendrobium officinaleKimura et Migo by an Alkaloid-knocking-out Method and theOn-line HPLC-UV-CL Analysis of Their Antioxidant ActivityCHEN Cun-wu1，2 ，CHEN Nai-dong1，2 ，3* ，MENG Yun-fei1 ，ZHANG Yan 1 ，HU Jian11 College of Biotechnology and Pharmaceutical Engineering，West Anhui University; 2 West Anhui BiotechnologyＲesearch Center of Natural Medicine and Traditional Chinese Medicine，West Anhui University，Anhui Lu’an 237012，China;3 Institute of Clinical Pharmacology，Anhui Medical University，Anhui Hefei 230032，ChinaAbstract: The aims of this study were to explore method for the on-line detection of alkaloids with antioxidant activityfrom the stems of Dendrobium officinale Kimura et Migo by HPLC-UV-CL system，and to provide references on researc- hing the potential material base of D． officinale and on quick discovery of antioxidants from traditional Chinese medicine． The on-line HPLC-UV-CL analysis with Luminol-perhydrol chemiluminescence system as the donor of hydroxyl radicals was applied to evaluate the antioxidant activity of the constituents of the total alkaloid extract from D． officinale． The al- kaloids were discerned by alkaloid knock out using phosphomolybdic acid and iodine-potassium iodide as precipitation reagent． It was resulted that total five kinds of D． officinale alkaloids ( HPLC peak 5，7，8，10 and 11) were detected and four of them showed remarkable hydroxyl-radical-cleaning activity wi