轮状病毒不同毒株感染宿主细胞的感染效力研究.pdfVIP

免疫学杂志第23卷第3期2007年5月IMMUNOLOGICALJOURNALV01．23No．3 ·255· May．2007 [文章编号]1000—8861(2007)03—0255—05 轮状病毒不同毒株感染宿主细胞的感染效力研究 何海洋1，李晋涛1，费 蕾1，姜 曼1，牟芝蓉1，付晓兰1，唐艳1，段召军2，吴玉章¨(1．第三军医 [摘要] 株病毒产量均随感染病毒用量增加而明显升高，而感染了SAll细胞的平均病毒生成能力显著高于感染wa株的细胞。 点提供了实验依据。 [关键词]轮状病毒；病毒感染率；病毒复制能力；流式细胞术；荧光集落分析法 [中图分类号]R373．9 [文献标识码]A studiesoftheinfectionofdifferentrotavirustohostcell Comparative HE Lei，JIANGMan，MOU Xiao—lan，TANGYah，DUAN Hai—yng，LIJin—tao，FEI Zhi—rong，FU Zhao—jun，WUYu—zhang Univer (IustituteImmunology，PM，ThirdMilitary 400038，China) of sity，Chongqing To the and inMAl04ofdifferentrotavirusstrainsWa [Abstract] ceHs and Objectivecompareinfectivityreproductivecapability their ininfection．MethodsThe was thevirustiters SAll，and differences mtavims differential were analyze preparedby centrifugation，and vims was detectedFFA．Flow wasusedtodetect toMAl04cells．ELISA todetectrotavirus amounts by cytometry infectivity employed particle and thedifferencesbetweenthetwomtavimsstrains．ResultsTheinfectiveofWatoMAl04cellswas than analyze ability obviouslyhigher thatofmtavirus thecellsinfectedSAllhave vires thanthoseinfectedWastrain．CondusionRotavirus— SAll，while by higherproductivity by esWaandSAl1has differentinfection resultsabasisfor the ofmtavimsinteractionwithhost obviously pattern．Thegive studyingproperties cells． wordsjRotavirus；Virus [Key infectivity；Virusreprod