00000129_癃开颗粒抗前列腺增生的药理毒理研究.docVIP

癃开颗粒抗前列腺增生的药理毒理研究 谢家骏1 钱伯初2 高崎1 周光兴3 蔡华芳2 乔正东1 成苗1 (1.上海市中药研究所，上海201203；2.浙江省医学科学院药物所，浙江 杭州310006；3.复旦大学，上海 200032) 关键词：癃开颗粒 前列腺增生 药效 毒理 小鼠 大鼠 犬 摘要：目的：探讨ig癃开颗粒抗前列腺增生药效与毒性作用。方法：给小鼠ig癃开颗粒，测定正常幼年小鼠和丙酸睾丸素皮下注射或尿生殖窦植入诱导的前列腺增生模型动物前列腺指数、前列腺组织内DNA含量、血清酸性磷酸酶活性、精囊和睾丸湿重。以口服给药途径和常规实验方法，检测单次给药的小鼠最大给药量，考察3个月连续给药的Wistar大鼠或Beagle犬长期毒性反应。结果：ig癃开颗粒20、40g/kg（Qd×20d）能降低正常幼年小鼠前列腺的重量及该组织内DNA的含量；ig癃开颗粒10、20、40g/kg（Qd×10d）能抑制丙酸睾丸素引起的小鼠前列腺腹叶的增生；ig癃开颗粒20、40g/kg（Qd×30d）能抑制植入胎鼠尿生殖窦引起的小鼠前列腺腹叶的增生；ig癃开颗粒100g/kg（Qd×13w）能使大鼠前列腺呈轻度萎缩状态，腺上皮由柱状向扁平移生，腺腔变小，停药4周未见消失；癃开颗粒ig对小鼠的最大给药量为200g/kg。癃开颗粒给大鼠ig10、40、100g/kg或给Beagle犬ig 12、60g/kg（Qd×13周），未发现有明显的毒性反应。结论：癃开颗粒口服能抑制前列腺增生且无明显毒性反应。 所属专业：应用药理（中药药理） 作者简介：谢家骏（1959～），男，教授级高级工程师。研究方向：药理毒理研究及中药新药开发。电话：021 Study on Pharmacodynamics and toxicoligy of Longkai Granules(LKG) against Prostatic Hyperplasia Xie Jiajun1 Qian Baichu2 Gao Qi1 Zhou Guangxing3 Cai Huafang2 Qiao Zhengdong1 Cheng Miao1(1.Shanghai Institute of Chinese Materia Medica,Shanghai 201203;2. Institute of Materia Medica,Zhejiang Academy of Medical Sciences, Zhejiang,Hangzou 310006;3.Fudan University, Shanghai 200032) Key words：Longkai Granules(LKG); Prostatic hyperplasia; Pharmacodynamics; Toxicology; Mice;Rats;dogs Abstract：Objective：To demonstrate the inhibitoary effects of LKC against experimental Prostatic hyperplasia and evaluate its toxicity on animals orally.Methods：The prostate exponent, DNA content in prostate tissue、the activity of acid phosphatase in serum or the wet weight of spermatophores and testicles in normal immature mice and in the hyperplasia model mice induced by subcutaneous injecting testooslerone spropionate or by implanting of the urogenital sinus were determined after administrating of LKG intragastrically to the mice.The single maximum dosage of LKG in mice and its long-term (13 weeks) toxicity in Wistar rats and Beagle dogs in orally was evaluated.Results：LKG can decrease the weights of prostates and DNA content in the tissue in the normal immature