鹅细小病毒的复制不依赖于宿主细胞的有丝分裂.pdfVIP

中国畜牧兽医学会禽病学分会禽流感及免疫抑制病论坛论文集 鹅细小病毒的复制不依赖于宿主细胞的有丝分裂丰 刘岳龙”李清金文杰陈希邵红霞秦爱建” (扬州大学兽医学院江苏扬州225009) 宿主细胞有丝分裂非依赖性的；此特性与细小病毒属的牛细小病毒相一致，而与其它细小病毒属的病毒不 同。 关键词：鹅细小病毒；复制；宿主细胞；有丝分裂 of iS on ofhostcells ReplicationgooseparvovirusindependentmitochysiS Liu Xi Ai—jian，ShaoHong—xia，SunZhi，Chen Yue—long，LiQing，JinWen—Jie，Oin of (CollegeVeterinaryMedicine，YangzhouUniversity，Yangzhou225009，China) Abstract：Goose strainwasthefirstisolatedstrainintheworld parvovirus(GPV)SYG61—53 wasattenuatedafter CZstrainwasisolated in1961and 53 in embryoes．GPV in passagesgoose 2004andwasavirulentstrainafter2 in 15 in passagesgooseembryoes．Afterpassagesgoose aboveGPV SYG61—53一cal5andCZ—cal5 embryofibroblasts(GEF)，twostrains(named to ofGEFwasblocked 36hrs hadbeen fibroblasts during post adapted sucssessfully．MitochysiS treated with C andColchicine (P．t．) mitochysiSinhibitor—Mitomycin andCZ—cal5strainsinoculatedintoabovemitostaticGEFandnormal GEF． SYG61—53一cal5 growing ofGPVinGEFweredetected anti—GPVmonoclonalantibodiesmediated Replications byspecial resultsoftwodifferent virusesinfected indirect—immunoflurescentassay(IFA)．The eell一adapted kindsofmitostatiCcellSandnormalmitoti