一株解烃菌TY12的分离及其特性研究.pdfVIP

Bioprocess 生物过程, 2015, 5, 6-13 Published Online March 2015 in Hans. /journal/bp /10.12677/bp.2015.51002 Study on the Identification and Characteristics of a Strain of N-Alkane Degrading Bacteria TY12 * Jia Tan, Yun Tang , Lijun Ji College of Biological Sciences, China West Normal University, Nanchong Shichuan * Email: tangyun_502@ st th th Received: Jan. 21 , 2015; accepted: Feb. 5 , 2015; published: Feb. 12 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract A strain of hydrophobic hydrocarbon degrading bacteria TY12 was screened out of petroleum contaminated soils from Nanchong oil refinery. Through 16S rDNA basic sequence detection and phylogenetic analysis, TY12 was identified as Serratia proteamaculans, and then according to morphological, physiology and biochemistry characteristics, this paper has a deep study on TY12. This paper studied on the cell-surface hydrophobicity of TY12 by microorganism adhering to hy- drocarbon (MATH), the results showed that the cell-surface hydrophobicity of TY12 was 52.2%, and the cell-surface hydrophobicity changed with the variation of incubation time. The n-dodecane degradation was 66.1% after TY12 was growing on in hydrocarbon degradation medium with 1% (W/V) of n-docosane at 28˚C and 180 r/min for 4 d. It had potential in bioremediation of oil con- taminated environment. Keywords Petroleum Pollution, Isolation and Identification, Alkane Biodegradation, Cell-Surface Hydrophobicity 一株解烃菌TY12的分离及其特性研究