支气管哮喘患者外周血,痰液中IL-33的含量及其相关性分析.docVIP

支气管哮喘患者外周血、痰液中IL-33的含量及其相关性分析 麻晓燕，罗雅玲，赖文岩，邵金莲，蓝海兵，梁振宇（510515广州，南方医科大学附属南方医院呼吸内科） [摘要] 目的 检测支气管哮喘患者外周血和痰液中IL-33的含量，探讨其含量与患者症状、肺功能、血液和痰液中嗜酸性粒细胞数的关系。方法 ELISA法检测80例支气管哮喘患者及20例正常对照者外周血中IL-33的含量，对哮喘患者行24 h症状评分和肺功能测试，依据FEV1将支气管哮喘患者分为3组：FEV1≥80%为轻度哮喘组，60%≤FEV1＜80%为中度哮喘组，FEV1＜60%为重度哮喘组，并记录哮喘患者外周血中嗜酸性粒细胞数目。ELISA法检测18例哮喘患者和8例正常对照者痰液中IL-33的含量，显微镜下计数其痰液中的嗜酸性粒细胞数。结果 外周血IL-33水平在正常对照组和轻度哮喘组之间无明显差异[（26.62±3.04）pg/ml vs（30.26±5.23）pg/ml，P﹥0.05]，但中、重度哮喘组的IL-33的水平要明显高正常对照组[（41.36±11.82）pg/ml vs（26.62±3.04）pg/ml，（84.10±20.17）pg/ml vs （26.62±3.04）pg/ml，P﹤0.05]，且与患者夜间症状评分呈正相关（r =0.317，P﹤0.05），而与白天症状评分和外周血嗜酸性粒细胞数无明显相关（r =0.173，P﹥0.05，r =0.082，P﹥0.05）,与患者FEV1和PEF均呈明显的负相关（r =-0.638，P﹤0.05；r =-0.552，P﹤0.05）。支气管哮喘患者诱导痰中IL-33的含量与痰液中嗜酸性粒细胞数目呈正相关(r =0.693，P﹤0.05)。结论 血液中的IL-33可作为一种评价哮喘患者肺功能下降程度的指标，是哮喘患者病情恶化的促进因子。 IL-33水平增高可能是诱导哮喘患者气道嗜酸性粒细胞浸润的原因之一。 [关键词]支气管哮喘；IL-33；外周血；诱导痰；相关性 [中图法分类号] [文献标志码] A The level of IL-33 in plasma of peripheral blood and sputum of bronchial asthma and its correlated analysis Ma Xiaoyan，Luo Yaling，Lai Wenyan，Shao Jinlian，Lan Haibing，Liang Zhenyu（Department of Respiratory,Nanfang Hospital,The Southern Medical University,Guangzhou 510515,China） [Abstract] Objective To measure the plasma and sputum level of IL-33 in asthmatic patients ,detect the relationship between plasma level of IL-33 and symptoms, lung function, blood eosinophil numbers ,and the relationship between the level of IL-33 and eosinophil numbers in sputum. Methods The plasma level of IL-33 in 80 patients with bronchial asthma and 20 normal control subjects was measured by enzyme-linked immunosorbent assay(ELISA). Their daytime and nighttime symptom scores were recorded respectively. Then the number of eosinophil in their peripheral blood was tested, and lung function was measured by Gaeger spirometry. According to FEV1 ,patients were divided into there groups: FEV1≥80% for the first group, 60%≤ FEV1＜80% for the second group, and FEV1＜60% for the third group. The sputum le