卵巢摘除及补充雌激素对小鼠骨折愈合中钙沉积的影响.docVIP

卵巢摘除及补充雌激素对小鼠骨折愈合中钙沉积的影响 卵巢摘除及补充雌激素对小鼠骨折愈合中钙沉积的影响 更新日期：2009-07-14 点击： 赵永强　王宏跃　李甲振　陈凤苞　刘志成 提　要　为研究雌激素对卵巢摘除后已发生显著骨丢失的balb/c系小鼠股骨骨折愈合的影响，将144只balb/c小鼠随机分为假手术组(SO组)、卵巢摘除组(OVX组)、补充低剂量雌激素组(E1组)、补充高剂量雌激素组(E2组)。小鼠于折骨后5天、10天、15天、20天、30天处死，测定骨痂中的钙盐沉积率。结果SO组、OVX组和E1组，骨痂中的钙盐沉积率在折骨后15天达峰值，OVX组钙盐沉积峰值显著低于SO组和E1组(P＜0.05)，后二者之间无显著差异。在E2组，骨痂中的钙盐沉积率峰值推迟至折骨后20天方出现。表明卵巢摘除的的低雌激素状态和补充超生理剂量的雌激素均对小鼠骨折愈合不利，补充生理剂量的雌激素有利于去势后小鼠的骨折愈合。 主题词　骨折愈合/药物作用　雌激素替代疗法　卵巢摘除　雌二醇/治疗应用　实验研究　动物　balb/c小鼠 中图分类号：R683；R977.1＋2　文献标识码：A　文章编号：1001-6015(2000) 05-0003-02 THE INFLUENCE OF OVARIECTOMY AND ESTROGEN SUPPLEMENT ON CALCIUM DEPOSITION IN MOUSE FRACTURE HEALING Zhao Yongqiang，Wang Hongyue，Li Jiazhen （Peoples Hospital of Henan Province, Zhengzhou 450003） In order to study the influence of estrogen on femoral fracture healing in balb/c mice with significant bone loss after ovariectomy, 144 balb/c mice were divided into Sham-operated (SO), ovariectomized (OV), low-dose-estrogen-treated (E1) and high-dose-estrogen-treated (E2) groups. The mice were killed at the 5th, 10th, 15th, 20th, and 30th day after fracture, respectively, and the calcium deposit rates in the callus (CDR) were measured. The results showed that CDR in SO, OV, and E1 groups reached the peak 15 days after the fracture; the CDR peak of OV group was much lower than those of SO and E1 groups (P 0.05), but there was no obvious difference between E1 and E2 groups in CDR. The CDR peak of E2 group was delayed and appeared 20 days after fracture, suggesting that both of the low estrogen and over-physiological-dose-estrogen supplement were harmful to mouse fracture healing, while the physiological-dose supplement of estrogen helps the fracture healing of ovariectomized mice. Key Words:　fracture healing/drug action　estrogen replacement therapy　ovariectomy　estrogen/therapeutic application　experimental study　animal　mouse 　　　 　　绝经后骨质疏松症(postmenopausal osteoporosis,PMO)是老年妇女的多发病，雌激素替代疗法(estrogen replacenent therapy,ERT)是防治PMO、减少骨折发生的有效措施〔1〕。PMO患者发生骨折后