有机相中脂肪酶催化合成乙酸芳樟酯的研讨.pdfVIP

有机相中脂肪酶催化合成乙酸芳樟酯的研究 1, 2 1, 2 2 2, ∗ 胡坤 ，韩亚杰 ，刘维英 ，代斌 （1. 石河子大学生命科学学院 石河子 832003 2. 新疆兵团化工绿色过程重点实验室 石河子 832003 ） 摘要：本研究首次以乙酸酐和芳樟醇为底物用脂肪酶催化法合成乙酸芳樟酯，考察了不同种类的商品脂 肪酶对该反应的催化效果，获得了一种对该反应催化效率较高的脂肪酶MML （Mucor miehei）,同时研究了 不同种类固定化载体对该脂肪酶活性的影响，确定了其最佳固定化条件。同时，考察了固定化后的脂肪酶 在有机相中催化合成乙酸芳樟酯的条件，确定其最佳反应条件：以异辛烷为最佳有机溶剂，以摩尔比为1:1.5 的芳樟醇和乙酸酐为底物，底物浓度为0.2mol/L，在33℃条件下，振荡反应24h，此条件下得到乙酸芳樟 酯的收率较高。通过该反应体系的建立，为今后工业生物催化法合成乙酸芳樟酯奠定良好基础。 关键词：有机相；乙酸芳樟酯；脂肪酶；固定化 A Research on linalyl acetate Catalyzed by Lipase in Organic Solvent 1, 2 1, 2 2 2∗ HU Kun HAN Ya-Jie LIU Wei-Ying DAI Bin (1 College of Life Science and Technology, Shihezi University, Shihezi, Xinjiang 832003 2 Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan Shihezi Xinjiang, 832003) Abstract: This research synthesizes a new ester by catalyzing substrate of acetic anhydride and linalool for the first time. It investigates the catalysis effect of different kinds of commercially available lipase, and gets a lipase MML (Mucor miehei) which can catalyze the reaction most efficiently. At the same time, it studies the effect which different kinds of fixed carriers have on the lipase, and finds out the best conditions for the fixation. Meanwhile, it explores the conditions in which the ester of acetic anhydride and linalool is catalyzed by the fixed lipase in organic solvent, and finds the best conditions for the reaction. They are: the best organic solvent must be isooctane; substrate of acetic anhydride and linalool should be 1:1.5 by Mole; the concentration of the