棉籽中脱叶剂噻苯隆的HPLC+法测定+研究.pdfVIP

H017 棉籽中脱叶剂噻苯隆的HPLC法测定 1，2 2 1 1、2 1 洪成林 ，马晓伟 ，孙世萍 ， 代斌 ，李秋梅 （1.石河子大学生物化工实验室,新疆石河子市, 832003； 2.石河子大学化学系，新疆石河子市, 832003） 摘 要 建立高效液相色谱 (HPLC)测定棉花脱叶剂噻苯隆 （thidiazuron ）残留量的检测方法。用 R 甲醇对棉花脱叶剂进行萃取分离并富集，用Symmetry○ C 5μm (4.6×150mm)色谱柱，甲醇－ 18 25mmol/L磷酸缓冲液 （44：56）为流动相，在290nm处进行紫外检测。结果表明最低检测浓度为 0.004μg/mL，棉籽中噻苯隆含量为0.037ppm，加样回收率为96.45-104.3%。 关键词 棉籽 棉花脱叶剂 HPLC含量测定 噻苯隆 THE DETERMINATION OF THIDIAZURON IN COTTONSEED BY HPLC 1,2 2 1 1,2 1 HONG Chenglin ，MA Xiaowei ， SUN Shiping ，DAI Bin ，LI Qiumei (1.Labortory of Biochemical Engineering , Shihezi University, Shi Hezi, 832003, China; 2.Department of Chemistry, Shihezi University, Shi Hez,832003, China) Abstract : To establish an analysis method using HPLC to determine thidiazuron, which is left over in Cottonseed. Thidiazuron was extracted from cottonseed with methanol. Using R Symmetry ○ C18 5µm Column(4.6×150mm), methanol-25mmol phosphate buffer (44:56) as mobile phase, thidiazuron w as eluted and monitored at 290nm. It was show n that the limit of determination was 0.004µg/mL, and the content of thidiazuron in the cottonseed was 0.037ppm , and the recovery of thidiazuron was 96.45-104.3%. Key words : Cottonseed; Defoliating agent; HPLC; Content determination; Thidiazuron [1] 噻苯隆 （thidiazuron ）是一种植物生长调节剂，大量用于机械采棉的脱叶剂 。化学名 为 N-苯基 N-1,2,3-噻二唑-5-脲 （C H N OS ）。对于噻苯隆的残留，美国环境保护局 9 8 4 (Environmental Protection Agency）在联邦公报(Federal Register )中规定棉籽及其它物质中