云南四个瓜实蝇地理种群的遗传关系分析-应用生态学报.PDFVIP

2005 10 16 10 CHINESE JOURNAL OF APPLIED ECOLOGY , Oct. 2005, 16( 10)∃1 9~ 1 92 * 1, 2 1* * 1 朱振华 叶 辉 张智英 1 2 ( , 650091; , 650205) 4 ( B actr ocer a c ucur bitae) 23 DNA b , ( B . dor salis) ( B . corr ect) ( B . tau) NJ . 426 bp , A+ T 650% , 4 , . 5 , . 4 Fst , Fst 0. 16667~ 0. 20000( P 0. 05) , Nm 2.00 ~ 2. 50. 5 3 . , 4 , , , . DNA b 1001- 9332(2005) 10- 1 9- 04 S 34 A Genetic relationships among four Bactr ocera cucurbitae geographic populations in Yunnan Province. ZHU 1, 2 1 1 1 Zhenhua , YE Hui , ZHANG Zhiying ( College of L if e Sc ience, Yunnan Un iv er sity , K unm ing 650091, Chi na 2I nst it ute of Cr op s , Yunnan A cademy of A g ricult ural Science, K unm ing 650205, China) . Ch in. J . A pp l . Ecol . , 2005, 16 ( 10) : 1 9~ 1 92. In this study , the partial sect ion of mitochondrial cytochrome b in 23 individuals of four B actr ocer a cucur bitae ge ographic populations( Banna, Ruili, Yuanjiang, and Hekou) in Yunnan Province was sequenced, and the phyloge netic trees of all haplotypes w ere constructed by means of NJ method, using B . dorsalis , B . cor r ect and B . ta u as the outgroup species. Of the 426 bp mitochondrial cytochrome b DNA sequences obtained, A% + T % was about 65. 0% , four nucleotide sites w ere substituted, and no base composition of the sequences w as inserted and lost. Five haplotypes were identified in all the sequences,w ith four show ing polymorphism, and one shared. The genetic characters of the four populat ions w ere analyzed by Fst value and gene flow through Mega software. The