接种用松材线虫分离种群的筛选.PDFVIP

1 1 1 1 2 高景斌 蔡卫兵 徐六一 席启俊 户田忠雄 ( 1 2 ) : 自2001年10 月以来开展了/ 马尾松松材线虫抗 育种技术的开发0 的项目工作, 为选拔马尾松松材线虫 抗 候补树,确保接种用的松材线虫虫源, 对松材线虫分离种群的繁殖致病力和增殖能力作了研究对不同地区 采取由松材线虫致死的19 棵当年枯死木样品,用贝曼漏斗法从中分离出19 个松材线虫分离种群,在25e 条件下, 成功培养16 个分离种群在2a 生黑松苗上进行接种, 测定松材线虫的致病力55d 后在野外试验区的感病率为 2611%~ 9716% , 大棚试验区的感病率为8414%~ 100% , 野外与大棚内的加权平均感病率差20% 以上, 显示出松材 线虫致病力由气温增高和土壤干燥而增强分别对16 个分离种群以相同的接种头数在PDA 培养, 其繁殖面积的 快慢不同; 以大量繁殖为目的, 在同量的小麦培养基中以相同的接种量, 各自的繁殖量也不同, 每培养皿的繁殖量 为98万~ 176 万头根据试验结果,选用致病力最强繁殖力较强的广朱3B 松材线虫分离种群作为接种用松材线 虫的接种虫源 :抗 育种;抽提物; 松材线虫;分离种群 Selection on Isolated Species for Bursap helenchus xylop hilus of Inoculation in Resistant- breeding 1 1 1 1 2 GAO Jing-bin , AI Wei-bing ,XU Lu-yi ,XI Q-i jun , TODA Ta-dao ( 1 Resistance Breeding entre for Pine Wood Nematode of Anhui Province;2 Forest Breeding entre of Japan Kyusyu Breeding entre)) Abstract : In order to select the wood nematode resistant alternative pines of P inus massoniana and to ensure the resources of the pinewood nematode used for inoculation, studies of the reproduction, infectious virulence and propagation have been done since the development of the project in October 2001. Nineteen wilted pine specimens died of nematode in different areas have been collected from which 19 nematode isolated species were separated by means of Baman and finally sixteen isolated species were cultivated successfully under the temperature of 25e . Then the two-years-old seedlings of pinus thunbergiiwere inoculated to examine the in- fective virulence of the pinewood nematode. The infective rates 55days later in thefield were between 26. 1%