16SrRNA基因序列分析技术在细菌分类中应用的研究进展_杨霞精选.pdf

36 2 ( ) Vol. 36 No . 2 2008 2 Journal of Northwest A F University( Nat. S i. Ed. ) Feb. 2008 16S rRNA * 杨 霞, 陈 陆, 王川庆 ( , 450002) [ ] 16S rRNA , 16S rRNA , [ ] 16S rRNA ; ; [ ] S852. 6 1 [ ] A [ ] 167 19387( 2008) Advance in applicat ion of 16S rRNA gene in bacteriology YANG Xia, CHEN Lu, WANG Chuanqing (Eng ineering College of A nima l H usbandr y and Veter inar y Sc ience,H enan A gr icultural Univ er sity , Zhengz hou, H enan 450002, Ch ina) Abstract : T he sequen e of 16S rRNA gene is highly onserved and univer sally distributed. T hus, as an ex ellent mole ule Marker, 16S rRNA is be oming a good tool for dete ting organism sms and their lassifi ation. In this paper, the hara ter of this gene, methods for studying and dete ting and lini appli ation, w ere review ed in details and the existing problems w ere inquired. Key words: 16S rRNA; mole ule marker; lassifi ation , , , , , PCR 16S rRNA [ 15] , , 16S r RNA , ( PCR) , , , PCR rRNA ( rD 1 16S rRNA NA ) 16S ( ribosomal RNA, rRNA) 16S rRNA RNA DNA , , , rRNA, , ( RN A 16S rRNA 80% ) , , *[ ] [ ] ( [ ] ( 1973- ) , , , , , [ ] ( 1953- ) , , , , , 56 ( ) 36 , , tan e M atr ix M ethods) ( Cluster Naly [ 6] , ; sis) , 6S rRNA , , [ 7]