基于g23基因的武汉东湖T4类浮游病毒遗传多样性 Genetic diversity of T4 virioplankton, inferred from g23 gene, in Wuhan Donghu Lake.pdfVIP

中国环境科学201 I，3l(3)：443州7 基于923基因的武汉东湖T4类浮游病毒遗传多样性 黄慧珍，程 凯，许 敏，赵以军。(华中师范大学城市水环境生态学湖北省重点实验室，湖北武汉430079) 摘要：选取武汉东湖的2个子湖——郭郑湖和庙湖，分别在冬季和夏季采样，对T4类浮游病毒的口3基因进行PCR扩增，经连接转化后，随 机挑选克隆子进行测序．结果表明，共得到46条有效序列，根据系统发育分析发现其明显分为6个组，显示出较高的多样性且具有明显的时 空差异．说明富营养化水平的差异和季节变化将对浮游病毒的种群结构产生影响．仅部分923序列与海洋T4类浮游病毒同源性较高，另一部 分序列则可能代表了淡水富营养化水体中特有的浮游病毒类群．人为干扰会明显影响某些淡水水体中T4类浮游病毒的遗传多样性． 关键词：923基因；东湖；T4：浮游病毒；遗传多样性 中围分类号：X172 文献标识码：A 文章编号：1000—6923(2011)03—0443-05 Genetic ofT4 from Wuhan Lake．HUANG diversity 923gene，in Donghu virioplankton，inferred Water ofUrban Environmental ChinaNormal Kai，XUMin，ZHAO1Ia-jLm’(HuboiKeyLaboratory Ecology,Central Environmental University,Wuhan Science，201l，3l(3)：443—147 430079，China)．China in andMiaohuLakewere inwinter Abstract：2sub-lakes Lake chosen,and Donghu sampledseperately Lake：Guozheng and ofT4 was certainclones．46 were summer．923gene virioplanktonamplified，randomlysequenced sequencesgot． showedthese fellinto6 andexihibited that independent Phylogeneticanalysis sequences groups high also levelandseason have todowiththe phylogeneticanalysissuggeststhat：1)theeutrophication cllangesomething structureof ofthe are relatedtomarineT4 the virioplanktoncommunity；21onlypart sequencesclosely others freshwater call influencethe mayrepresenteutrophicatedvirioplankton；31anthropogenicforcingobviously genetic ofT4 incertainfreshwaters． diversi