聚丙烯酸水滑石纳米复合凝胶的制备及其碳酸酐酶的固定化研讨.pdfVIP

聚丙烯酸/水滑石纳米复合凝胶的制备 及其碳酸酐酶的固定化研究 张亚涛 范立海 张 林 陈欢林∗ （浙江大学材料与化学工程学院，杭州310027 ） 摘 要：本文采用甲基丙烯磺酸钠(SMAS)作插层剂，通过原位聚合制备了聚丙烯酸/水滑石(PAA/HT)纳米 复合凝胶。在二环己基碳二亚胺(DDC) 的存在下，用 N-羟基琥珀酰亚胺(NHS)对凝胶进行活化，详细研究 了该凝胶对碳酸酐酶(CA)的固定化。通过傅立叶变换红外光谱(FTIR)、X-射线衍射(XRD)及扫描电镜(SEM) 等手段表征其结构和形貌。结果表明插入SMAS 的水滑石片层在聚合后发生了剥离，添加少量的SMAS-HT 可以明显提高凝胶的吸(盐)水性能，当SMAS-HT 含量为3.0wt%时，凝胶的吸(盐)水性能达到最大。最后比 较了游离酶和凝胶固定化酶的CO2 水合活性，凝胶固定化的酶活回收率为76% ，而且凝胶固定化酶的热稳 定性有所提高，60 min 后，固定化酶的活性可以保持在最初活性的80% 以上，表明该凝胶是一种很好的酶 固定化载体材料。 关键词：纳米复合凝胶；固定化；碳酸酐酶 Poly (acrylic acid)/ hydrotalcite (PAA/HT) nanocomposite hydrogels: preparation, and immobilization studies of Carbonic Anhydrase ZHANG Yatao, ZHANG Lin, CHEN Huanlin (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China) Abstract: Poly (acrylic acid)/hydrotalcite (PAA/HT) nanocomposite hydrogel was prepared by in-situ polymerization, using methyl allyl sulfonate (SMAS) as an intercalation agent. Carbonic Anhydrase (CA) was immobilized on the nanocomposite hydrogel activated by N-hydroxysuccinimide (NHS) in the presence of N,N’-dicyclohexylcarbodiimide (DCC).The structure and morphology were characterized by FTIR, XRD, and SEM. The results show that the intercalation SMAS-HT is completely exfoliated after polymerization and adding a small amount of SMAS-HT can effectively improve the water (salt) absorbency of nanocomposite hydrogels. The nanocomposite hydrogels acquire their highest water (salt) absorbency when the content of ∗ 联系人：张林，E-mail: linzhang@zju.edu.cn ，国家自然科学基金资助项目（No.） SMAS-HT is 3.0 wt%. The CO hydration activities of the free CA and CA immobilized on the 2 nanocomposite hydrogel were compared. Results showed that PAA/HT nanocomposite hydrogel was a promising kind of carrier for enzyme im