转AcAMP-sn基因抗全蚀病小麦新种质的创制与鉴定.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2014, 40(1): 22−28 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.00022 转AcAMP-sn 基因抗全蚀病小麦新种质的创制与鉴定 杨 坤 刘 欣 杜丽璞 叶兴国 张增艳* / / , 100081 : , AcAMP-sn , pAHC25::AcAMP-sn, AcAMP-sn Ubiqutin , AcAMP-sn 18, PCR RT-PCR RT-PCR T0~T4 , PCR , AcAMP-sn 18 , 5 T4 , AcAMP-sn , , AcAMP-sn : ; ; ; Development and Characterization of AcAMP-sn Transgenic Wheat with En- hanced Resistance to Wheat Take-all * YANG Kun, LIU Xin, DU Li-Pu, YE Xing-Guo, and ZHANG Zeng-Yan National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China Abstract: Antimicrobial peptides, small molecular weight proteins with broad spectrum antimicrobial activity, play important roles in plant defense responses. The open-reading-frame sequence of AcAMP-sn was synthesized and used to construct the gene transformation vector pAHC25::AcAMP-sn, in which AcAMP-sn gene was driven by maize ubiquitin promoter and should be highly expressed in monocot plants. The vector pAHC25::AcAMP-sn DNA was introduced into Yangmai 18 via particle bo