rnai-2006诺贝尔生理学奖或医学奖.pptVIP

最初由Harvard医学院教授构建并成功运用于knock down expression of cdk-2 and lamin A/C in HeLa, H1299, U-2 OS and C-33A (cdk-2 only) 细胞中 Protocol与环状结构模式图 most of the groups developed plasmids with a sequence encoding a small hairpin RNA (shRNA) under the control of an RNA Polymerase III (Pol III) promoter. The U6 and H1 Pol III promoters were chosen because Pol III initiates synthesis at a defined distance from these promoters and terminates when a string of 4-5 uridines is encountered. When transfected into mammalian cells, siRNA expression plasmids and have been shown to reduce the levels of both exogenous and