repPCR对赤扬根瘤内Frankia菌基因多样性研讨.pdfVIP

rep-PCR对赤杨根瘤内Frankia菌基因多样性研究 摘 要 本文应用重复基因外回纹序列 rep-PCR方法直接扩增长白山三种不同赤杨 和云南不同地区早冬瓜根瘤内的Frankia菌基因，所获得的REP指纹图谱揭示 Frankia菌具有丰富的基因多样性。研究结果表明Frankia菌多样性随地域，海 拔，坡向不同而变。多样性指数与自然选择压力大小成正相关。自然选择压力是 产生和保持Frankia菌基因多样性的重要因子。同时发现 Frankia菌基因型与地 域有紧密关系。所观察的云南各地区中，感染高黎贡山旱冬瓜的Frankia菌基因 多样性最为丰富。推测与高黎贡山旱冬瓜共生的Frankia可能作为种储备库，为 其它地区的寄主植物提供了祖先菌株。 在对长白山三种赤杨 (东北赤杨，西伯利亚赤杨和色赤杨)根瘤内Frankia 菌基因多样性考察过程中发现，Frankia菌基因类型与寄主赤杨种型关系密切。 同时海拔对感染东北赤杨的Frankia菌基因多样性也有一定影响。感染西伯利亚 赤杨和色赤杨的Frankia菌享有相同的基因型，可能有较近的亲缘关系。 关键词:赤杨，基因多样性，Frankia, Abstract TheStudyonGeneticDiversityofFrankiaStrainsinthe nodulesofAInusbyrep-PCRtechnique ThegeneticdiversityoftheAlnus-infectiveFrankiastrainswas studiedbyrep-PCR(RepetitiveExtragenicPalindromic)technique.The genomicDNAs,isolatedfromFrankiastrainsinthenodulesofAlnus,were amplifieddirectly.ThesamplescollectedarethoseFrankiastrains symbiosingwithAlnusnepalensisinYunnanProvinceandwiththreespecies ofAlnusgrowinginChangbaiMountain,whichareAlnustinctoria,A. mandshuricaandA.sibiricarespectively.TheresultingREPfingerprint patternsobtainedrevealthatFrankiahasanabundantgeneticdiversity. ThediversityofFrankiastrainsgrowingindifferenthabitatsofJizu andWuliangMountainsvarieswithdifferentzones,altitudesandslopes. Thediversityindexispositivelyrelatedtonaturalstress,andthelatter isthemainfactoraffectingtheproduceandmaintenanceoftheFrankia geneticdiversity.ForalloftheregionssampledinYunnanProvince, genotypesappeartobeendemictotheirregionsexceptforthosefrom GaoligongMountain,inwhichFrankiahasthemostgenotypesandhighest diversityindexamongthefiveregions.ItisconcludedthatFrankia strainssymbioticallyassociatedwithAInusnepalensisgrowing in GaoligongMountainperhapsarethegeneticstoreroom,whichoffersother actinorhizalplantshabitatinginotherregionsancestorstrains. DuringstudyingthegeneticdiversityofFrankiastrainssymbiotically associatedwithAln