响应曲面法优化小黄鱼ACE抑制肽的酶解条件.pdfVIP

※生物工程 食品科学 2011, Vol. 32, No. 23 16 7 响应曲面法优化小黄鱼ACE 抑制肽的酶解条件 蔡丽丽，徐 鑫* ，刘国艳，何佳易，魏晓蕊，王之颖 (扬州大学食品科学与工程学院，江苏 扬州 225 127) 摘 要：以小黄鱼为原料，利用酶解技术制备高活性的ACE 抑制肽。通过单因素及响应曲面分析，确定胰蛋白 酶(PTN6.0S) 的酶解工艺，利用反相高效液相色谱(RP-HPLC)法测定酶解产物(0.05g/L) 的ACE 抑制率，研究pH 值、 温度、时间、底物质量浓度、酶与底物比等因素对A C E 抑制肽活性的影响。结果显示：最佳工艺条件为 p H7.0 、温度50 ℃、时间16.5h 、底物质量浓度5.6g/ 100mL 、酶与底物比9‰，酶解产物ACE 抑制率达87.36% 。 关键词：小黄鱼；胰蛋白酶；A CE 抑制肽；响应曲面法；反相高效液相色谱(RP -HP L C) Optimization of Hydrolysis Conditions for Preparation of Small Yellow Croaker-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides Using Response Surface Methodology CAI Li-li ，XU Xin *，LIU Guo-yan ，HE Jia-yi ，WEI Xiao-rui ，WANG Zhi-ying (College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China) Abstract ：In this study, small yellow croaker meat was hydrolyzed by trypsin (PTN 6.0S) to prepare angiotensin-I converting enzyme (ACE) inhibitory peptides with high activity. Using one-factor-at-a-time method and response surface analysis (RSA), the optimal hydrolysis conditions for maximum ACE inhibitory rate of hydrolysates were determined as follows: pH 7.0, 50 ℃, 16.5 h hydrolysis time, 5.6 g/ 100 mL substrate concentration, and 9‰enzyme/substrate ratio. The ACE inhibitory rate of the hydrolysate obtained under these conditions was determined by RP-HPLC to be 87.36%. Key words ：small yellow croaker ；tryp sin ；ACE inhibitory peptides ；response surface methodology ；RP-HPLC 中图分类号：TS2 18 文献标识码：A 文章编号：1002-6630(20 11)23-0 167-05 [ 16 ] 血管紧张素转化酶A CE (an g i ot en sin c on v er t in g 液质联用法 分析成本较高，不便普及使用。本研究