IP6对人结肠癌细胞HT.docVIP

IP6对人结肠癌细胞HT29凋亡Caspase3基因作用 【摘要】 目的 研究肌醇六磷酸（IP6）对人结肠癌细胞HT29的诱导凋亡作用，检测Caspase3基因在IP6处理细胞前后表达的变化，并对IP6诱导人结肠癌细胞HT29的凋亡机制进行初步探讨。方法 不同浓度的IP6以不同时间作用于体外培养的HT29细胞，用MTT法检测IP6持续作用对癌细胞增殖的影响；透射电镜观察癌细胞超微结构的变化；用RTPCR方法对IP6作用前后以及作用不同浓度和时间的癌细胞内Caspase3活性进行检测。结果 IP6能显著抑制人结肠癌细胞HT29生长，呈剂量与时间依赖性；IP6作用后，HT29细胞出现凋亡的形态学改变；与对照组相比，不同浓度的IP6作用不同时间后，Caspase3 mRNA表达均升高（F=8.173～91.755,q=3.960～17.095，P0.05）, IP6作用6个月组Caspase3 mRNA表达升高较其他时间组显著（q=12.858～14.890，P0.01）。结论 IP6具有抑制人结肠癌细胞HT29增殖和诱导凋亡的作用；IP6长期作用诱导细胞凋亡更加显著；Caspase3基因参与细胞的凋亡调节机制，发挥重要作用。 【关键词】 植酸 HT29细胞 细胞凋亡 基因 Caspase3 　　IP6 INDUCES APOPTOSIS AND ENHANCES EXPRESSION OF Caspase3 IN HT29 HUMAN COLON CARCINOMA CELL LINE　SONG LI, SONG YANG　(Institute of Nutrition, Qingdao University Medical College, Qingdao 266021, China); 　　［ABSTRACT］ Objective To study the mechanisms of apoptosis induced by Inositol Hexaphosphate (IP6) in HT29 human colon carcinoma cell line. Methods HT29 cells cultured in vitro were exposed to various concentrations of IP6 applied at different time. The constant effect of IP6 on HT29 cells proliferation was evaluated by MTT assay. The morphological changes of HT29 cells were observed under electronic microscope.The activity of Caspase3 in HT29 cells was detected by RTPCR assay. Results IP6 remarkbly suppressed the growth of HT29 cells. The suppression was a dosedependent and timedependent manner. HT29 cells showed morphological changes of apoptosis after IP6 treatment.Compared with the control group, the expression of Caspase3 mRNA was significantly increased at all IP6 concentrations (F=8.173-91.755,q=3.960-17.095,P0.05). The upregulation of Caspase3 mRNA expression was significantly higher in the 6month group than the rest groups (q=12.858-14.890,P0.01). Conclusion IP6 can suppress the proliferation and induce the apoptosis of HT29 cells. Longer IP6 treatment induced stronger apoptosis. Caspase3 plays an important role in the regulatory mechanisms of cells apoptosis. ［KEY WORDS］ Phytic acid; HT