长非编码MALAT1基因在人鼻咽癌细胞株的表达及生物学意义-Open.PDFVIP

基础研究 长非编码长非编码MALATMALAT11基因在人鼻咽癌细胞株的表达及生物学意义基因在人鼻咽癌细胞株的表达及生物学意义 1，2 2 2 1，2 谢林英 ，胡志燕 ，王晓燕 ，李祖国 1 2 南方医科大学 南方医院病理科，基础医学院病理学教研室，广东 广州 510515 摘要：目的探讨MALAT 1在鼻咽癌细胞细胞株中的表达及生物学功能。方法 通过Real-time PCR 检测MALAT 1基因在鼻炎 癌细胞株5-8F、C666-1、CNE-1、CNE-2、HONE- 1、6-10B 及永生化鼻咽上皮细胞NP69 株中的表达；采用RNAi 技术和RNA 激活 技术，构建MALAT 1慢病毒干扰载体和激活载体载体并稳定转染鼻咽癌细胞株CNE-1，采用CCK8、平板克隆、划痕等试验分析 MALAT 1基因对CNE-1细胞生物学行为的影响。结果 MALAT 1在高转移的鼻咽癌细胞株中高表达，在正常鼻咽上皮细胞低 表达；经激活过表达MALAT 1后，CNE-1细胞的上皮性标志物E-Cadherin 的表达显著下调，间质细胞标志物Vimentin 表达上 调，侵袭转移能力明显增强（P0.05）。结论 MALAT 1基因上调能够促进鼻咽癌CNE-1细胞的增殖、侵袭和转移能力。 关键词：MALAT 1；鼻咽癌；基因表达；生物学功能 Expression of long noncoding RNA MALAT1 gene in human nasopharyngeal carcinoma cell lines and its biological significance 1, 2 2 2 1, 2 XIE Linying , HU Zhiyan , WANG Xiaoyan , LI Zuguo 1 2 Department of Pathology, Nanfang Hospital , Department of Pathology , Southern Medical University, Guangzhou 510515, China Abstract: Objective To explore the expression of MALAT 1 in nasopharyngeal carcinoma (NPC) and its biological function. Method Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-1, 6-10B and NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference (RNAi) and RNA activation (RNAa) techniques were examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, Transwell in vi- tro invasion assay and wound-healing assay ability. Results MALAT1 was highly expressed in 5-8F cells with a high metastat- ic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa sup- pressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation,