冷冻电镜.ppt.pptxVIP

cryo-electron microscopy and structural biology（冷冻电镜与结构生物学）制作 :丁宇航组员：谭志洪 张康 吴京洋 苟雪莲 刘雄 朱瑜我们或许在不久的将来就能获得生命复杂机制的原子级分辨率的图片了。这正是 2017 年的诺贝尔化学奖，授予 Jacques Dubochet, Joachim Frank 和 Richard Henderson，因他们在冷冻电子显微镜方面的卓越贡献，他们将冷冻电子显微镜技术简化，并将其应用在生物分子成像方向。此种成像技术将生物化学领域推进了新时代。The History of Application of cryo-Electron Microscope in Structural Biology目录The process and working principle of cryo - electron microscopy?The advantages and limitations of cryo-Electron Microscope?Application of cryo-Electron Microscope in Modern BiologyContentsWhat is frozen electron microscope?cryo-electron microscopy: the use of frozen fixed technology, low temperature using a transmission electron microscope to observe the sample micro-technology, resulting in the structure of biological macromoleculesHistory: In the 1970s, the structure of the virus molecule was studied by using freeze electron microscopy, and the concept, method and concept of the method of freezing electron microscope were put forward for the first time（20世纪70年代通过利用冷冻电镜研究病毒分子的结构，从而首次提出冷冻电镜技术的原理，方法以及流程的概念)90 years of the 20th century with the frozen transmission device, field emission electron gun and CDD imaging device appears, frozen electron microscopy single particle technology.（20世纪90年代随着冷冻传输装置、场发射电子枪以及CDD成像装置出现，冷冻电镜单颗粒技术）the early 20th century to the development of ergodic virus single particle three-dimensional reconstruction technology.（20世纪初期发展至二十面体病毒的单颗粒三维重构技术）In 2010, the three-dimensional reconstruction technique of cryo-electron microscopy was used to determine the structure of protein TRPV1, which marked that the cryostat was involved in the era of atomic resolution（2014年利用冷冻电镜三维重构技术确定蛋白质TRPV1结构，标志着冷冻电镜跨入“原子分辨率”时代）How the cryo-electron microscope works:principle:?The sample is fixed by freezing in liquid nitrogen, making the biology large H2O molecules in the form of glass in the form of existence, to maintain low temperature,The sample is placed in a microscope, highly coherentelectrons as a light source from th