子宫颈癌中HIF1α的表达和血管生成的关系研究.pdfVIP

中 文 摘 要 目的:探讨子宫颈鳞癌及癌前病变 (CINIII)中缺氧诱导因子一Ia(HIF-1a), 和微血管密度 (MVD)的表达，研究两者之间的相互关系及临床意义。方法:应用 免疫组化方法检测HIF-1。的表达，用CD,,单克隆抗体标记肿瘤MVD。结果:宫 颈癌组HIF-1a阳性表达率为80%，明显高于正常对照组 16.7% (P0.01);癌前病 变CINIII组阳性表达率为71.4%，与宫颈癌组和正常对照组均无显著差异。宫颈癌组 的MVD为6.34士1.96，高于正常对照组的2.33士1.03(P0.01)aCIN组的MVD为 5.54士2.39与正常对照组有显著差异，而与宫颈癌组无显著差异。HIF-Ia的表达与 宫颈癌的病理分级密切相关 (r5=0.47P0.05)，而与临床期别、淋巴结转移无明显 相关性。此外，HIF-I。的表达与MVD呈正相关 ((r,=0.71P0.05)。结论:宫颈癌 HIF-1a的过度表达与肿瘤血管生成密切相关，HIF-1a的过度表达随宫颈癌的恶 J胜程度增加而增高。 关键词:缺氧诱导因子一1a (HIF-1a);微血管密度 (MVD);子宫颈鳞癌; CINIII Theassociationbetweenexpressionofhypoxia-induciblefactor-la and angiogenesisincervicalcancer, ABSTRACT: OBJECTIVE:Toexploretheassociationbetweentheexpressionofhypoxia-inducible factor-la(HIF-la)andthemicrovesseldensity(MVD)incervicalcancerandits precancerosis,andtoanalyzetheclinicalsignificanceofthem.METHODS:Theexpression ofHIF-laandmicrovesseldensitywasdetectedbyimmunohistochemicalmethods. RESULTS:Incervicalcancer,thepositivepercentofHIF-la(80%)wassignificantmore thanthenormaltissues(16.7%，p0.01)，thepositivepercentofHIF-lainprecancerosis CINIIIis71.4%，comparedwiththoseinthecancertissuesandnormaltissues，therewere nosignificantdifferences.ThelevelofMVDincervicalcancerwassignificantmorethan thatinthenormaltissues(6.34士1.96VS2.33t1.03，p0.01).InCNIIII，thelevelof MVDwas5.54t2.39，hadsignificantdifferencewithnormaltissues，buthadno differencewithcancertissues.Furthermore，theexpressionofHIF-lawassignificant associatedwiththehistologicalgrading(rs=0.47，p0.05)buttherewerenorelatedwith thestageofcervicalcancerandthelymphaticnodemetastasis.Inaddition，theexpression ofHIF-lahadthepositiverelationshipwiththeMVD (rs=0.71，p0.05). CONCLUSIONS:OverexpressionofHIF-1aincervicalcancerseemstobecloselyrelated withneoangiogenesis，andthelevelofitisincreasedwiththedegreeoftumormalignancy added. KEYWORDS:HIF-la MVD cervicalcancer CINIII 文 献 综 述 恶性肿瘤以细胞无法调控的增殖为最主要