三维荧光光谱法分类测量水体浮游植物浓度 The classified measuring of three dimensional excitation-emission fluorescence matrix technique on phytoplankton concentration in water body.pdfVIP
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三维荧光光谱法分类测量水体浮游植物浓度 The classified measuring of three dimensional excitation-emission fluorescence matrix technique on phytoplankton concentration in water body
China Science
中国环境科学2008,28(2):136~141 Environmental
三维荧光光谱法分类测量水体浮游植物浓度
230026:2.中国科学院安徽光学精密机械研究所,中国科学院环境光学与技术重点实验室,安徽合肥23003I)
摘要:测量了富营养化水体中13种浮游植物的活体三维荧光光谱和激发荧光光谱。通过光谱相似性原则将浮游植物分成3种光谱组,分析
了光合色素组成与各光谱组三维荧光光谱特征的关系.选择梅尼小环藻、小球藻和铜绿微囊藻纯种培养体,配制lO种不同浓度比例的浮游
尼小环藻)、组分2(小球藻)和组分3(铜绿微囊藻)在模I上得分与其在混合样品中的实际相对浓度具有良好的线性关系,相关系数分别达到
0.98222.0.97865和0.99788.证实了利用三维荧光光谱和PARAFAC模型分类测量浮游植物浓度的可行性.
关键词:浮游植物:三维荧光光谱:平行因子模型;活体荧光
中图分类号:X824 文献标识码:A 文章编号:1000--6923(2(308)02-0136-06
Theclassified ofthreedimensionalexcitation-emissionfluorescencematrix Oil
measuring technique
in
concentrationwater
body.WANG Yu-jun2,YIN Jian·gu02
Zhi.gan91·2,LIUWen.qin92’,ZHANG Gao—fan92,LIU
Earth of of
of and Scienceand China,Hefei
(1.School Science,UniversityTechnology
Space
Instituteof of
ofEnvironmentaland andFineMechanics,Chinese
Laboratory OpticsTechnology,AnhuiOptics Academy
Sciences,Hefei230031,China).ChinaEnvironmentalScience,2008,28(2):136-141
excitation-emission excitationfluorescenceof13
Abstract:Threedimensional fluorescence
spectra(3DEEM)and spectra
kindsof in waterweremeasured.The weresortedtOthreekindsof
cultures
phytoplanktoneutrophied phytoplanktons
betweenthe of andthe
spectralgroupthroughspectrumsimilarityprinciple.Relationcompositionphotosynthesispigment
threedimensionalfluorescencecharacteristicofeach WaS cultur
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