崔银秋-分子生物学-CHAPTER 11 Site-Specific Recombination Transposition of DNA.pptVIP

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崔银秋-分子生物学-CHAPTER 11 Site-Specific Recombination Transposition of DNA.ppt

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Site-Specific Recombination Transposition of DNA Genome Conservative Site-Specific Recombination Biological Roles of Site-Specific Recombination Transposition Examples of Transposable Elements and Their Regulation Conservative Site-Specific Recombination Biological roles of site-specific recombination Many phage insert their DNA into the host chromosome during infection using this recombination mechanism. Example: λphage Alter gene expression. Example: Salmonella Hin recombinase Maintain the structural integrity of circular DNA molecules during cycles of DNA replication. Example: resolvase that resolves dimer to monomer l integrase Excision integration l genome integration. Recombination always occurs at exactly the same sequence within two recombination sites, one on the phage DNA, and the other on the bacterial DNA. Recombinase: λInt / Xis: tyrosine recombinase IHF: intergration host factorRecombination site: att B: Int binding site att P: C--- Int binding site H---IHF binding site X---Xis binding site P--- Int binding site Staggered cleavages in the common core sequence of attP and attB allow crosswise reunion to generate reciprocal recombinant junctions. Biological roles of site-specific recombination Many phage insert their DNA into the host chromosome during infection using this recombination mechanism. Example: λphage Alter gene expression. Example: Salmonella Hin recombinase Maintain the structural integrity of circular DNA molecules during cycles of DNA replication. Example: resolvase that resolves dimer to monomer The Hin recombinase The Salmonella Hin recombinase inverts a segment of the bacterial chromosome to allow expression of two alternative sets of genes, which is known as an example of programmed rearrangements common in bacteria. Hin inversion is used to help th