组蛋白与材料协同效应-p53 gene delivery system for tumor targeted transfection.pdfVIP

Biomaterials 31 (2010) 4771e4780 Contents lists available at ScienceDirect Biomaterials journal homepage: www.elsevi /locate/biomaterials Biotinylated transferrin/avidin/biotinylated disulfide containing PEI bioconjugates mediated p53 gene delivery system for tumor targeted transfection * Xuan Zeng, Yun-Xia Sun, Wei Qu, Xian-Zheng Zhang , Ren-Xi Zhuo Key Laboratory of Biomedical Polymers of Ministry of Education Department of Chemistry, Wuhan University, Wuhan 430072, PR China a r t i c l e i n f o a b s t r a c t Article history: As mutation and dysfunction of p53 gene could induce most of human cancers, the p53 tumor suppressor Received 3 February 2010 gene was used to replace them and recover their normal functions in cancer cells. In this paper, bio- Accepted 12 February 2010 tinylated transferrin/avidin/biotinylated disulfide containing PEI bioconjugates (TABP-SS) mediated p53 Available online 9 March 2010 gene delivery system was formed attributed to the ‘avidinebiotin bridge’. Characteristics of the obtained TABP-SS and its p53 complexes were evaluated in terms of acidebase titration, agarose gel electrophoresis, Keywords: SEM, particle size and z-potential measurements. The acidebase titration results showed that TABP-SS had Gene vector good buffer capabi