DBD等离子体合成羧基功能薄膜及其细胞吸附性研讨.pdf

2008 全国荷电粒子源、粒子束学术会议论文集 DBD等离子体合成羧基功能薄膜及其细胞吸附性研究 胡文娟 1，陈强 1*，谢芬艳 1,2，蔡惠平 1 1-北京印刷学院印刷包装材料与技术北京市重点实验室，北京大兴，102600 2-东莞理工学院，广东东莞，523808 摘要：本文使用介质阻挡放电装置，以丙烯酸为反应单体，在不同的基材表面聚合羧基功能 薄膜。利用接触角测量仪，傅立叶变换红外光谱(FTIR)，原子力显微镜(AFM)，X 射线光电 子能谱（XPS ）对功能薄膜的性能、结构、成分以及表面形貌进行测试表征。通过体外细胞 培养和富血血小板黏附实验，讨论了含有羧基功能团薄膜的结构、成分以及性能对其生物相 容性的影响，对功能薄膜与细胞之间相互作用进行了初步的研究。实验表明：等离子体放电 参数，例如电源频率、工作气压、功率、占空比等，对合成羧基功能薄膜的结构与成分有较 大的影响，且聚合的羧基功能薄膜能较好地促进细胞在其表面的吸附以及生长。 关键词：DBD 放电，羧基薄膜，占空比，细胞吸附 * chenqiang@bigc.edu.cn, Tel: 010-6026-1099, Fax: 010-6026-1108 Acrylic Acid functional films polymerized by DBD and its cell adsorption behaviors 1 1* 2 1 Wenjuan Hu , Qiang Chen , Fenyan Xie , Huiping Cai 1- Laboratory of Plasma Physics and Materials, Beijing Institute of Graphic Communication, 102600, Beijing, China; 2- Dongwan Institute of Technology, 523808, Guangdong, China Abstract ：Plasma polymerized acrylic acid (PPAA) coatings are carried out by using acrylic acid as a precursor in a dielectric barrier discharge (DBD). The film properties have been characterized by water contact angle, Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). By in-vitro cell culture and rich blood platelet adsorption tests, the influence of film structures, chemical components as well as its biocompatibilities were studied. The biological effect of functional coatings was also discussed in detail. The results show that plasma parameters, such as source frequency, plasma power, discharge mode, gas pressure, duty cycle, have great influence on PPAA coatings, and the very efficient cell adsorption behaviors was demonstrated on PPAA coatings surface. Key words ：pulsed dielectri