Disruption+of+Homocysteine+Methyltransferase+MET6+Gene+to+Enhance+Glutathione+Content+in+Saccharomyces+cerevisiae研究.pdf

Disruption+of+Homocysteine+Methyltransferase+MET6+Gene+to+Enhance+Glutathione+Content+in+Saccharomyces+cerevisiae研究.pdf

  1. 1、本文档共18页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Disruption of Homocysteine Methyl-transferase (MET6) Gene to Enhance Glutathione Content in Saccharomyces cerevisiae Zheng Wang, Zhao Zhao, Tianwei Tan* Beijing Key Lab of Bioprocess ,College of Life Science and Technology ,Beijing University of Chemical Technology,Beijing 100029 ,P.R. China * Author for correspondence (Fax:86-010 E-mail address:twtan@) ABSTRACT In an attempt to improve glutathione content in Saccharomyces cerevisiae, the haploid strain (a) was constructed by genetic homologous recombination, in which the homocysteine methyl-transferase (MET6) gene was disrupted. The mutant strain was tested on 5 l fermentor compared with that of the parent strain, and the results showed an obvious increase of glutathione content. The final glutathione yield of the mutant reached 1656 mg/l with 40% increasement compared to the parent. Therefore, these approaches uncover the possibility of knocking out MET6 gene and increasing glutathione yield during fermentation. Keywords: disruption, glutathione, haploid, Saccharomyces cerevisiae, Introduction Glutathione (GSH), ubiquitous in cells, functions as an antioxidant against the toxic effects of O2 and other oxidative compounds. Also, this active tripeptide possesses multifunctional properties, such as an important biochemical drug for the treatment of liver diseases and an important cofactor widely used by biochemical scientists. So, it was used as a pharmaceutical compound, food additives as well as the cosmetic ingredient and elicits more interest in recent studies. GSH is synthesized in two ATP-dependent steps (Meister and Anderso

您可能关注的文档

文档评论(0)

带头大哥 + 关注
实名认证
内容提供者

该用户很懒,什么也没介绍

1亿VIP精品文档

相关文档