Disruption+of+Homocysteine+Methyltransferase+MET6+Gene+to+Enhance+Glutathione+Content+in+Saccharomyces+cerevisiae研究.pdf
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Disruption of Homocysteine Methyl-transferase (MET6) Gene to
Enhance Glutathione Content in Saccharomyces cerevisiae
Zheng Wang, Zhao Zhao, Tianwei Tan*
Beijing Key Lab of Bioprocess ,College of Life Science and Technology ,Beijing University of Chemical
Technology,Beijing 100029 ,P.R. China
* Author for correspondence (Fax:86-010 E-mail address:twtan@)
ABSTRACT
In an attempt to improve glutathione content in Saccharomyces cerevisiae, the haploid strain (a)
was constructed by genetic homologous recombination, in which the homocysteine
methyl-transferase (MET6) gene was disrupted. The mutant strain was tested on 5 l fermentor
compared with that of the parent strain, and the results showed an obvious increase of glutathione
content. The final glutathione yield of the mutant reached 1656 mg/l with 40% increasement
compared to the parent. Therefore, these approaches uncover the possibility of knocking out
MET6 gene and increasing glutathione yield during fermentation.
Keywords: disruption, glutathione, haploid, Saccharomyces cerevisiae,
Introduction
Glutathione (GSH), ubiquitous in cells, functions as an antioxidant against the toxic effects of O2
and other oxidative compounds. Also, this active tripeptide possesses multifunctional properties,
such as an important biochemical drug for the treatment of liver diseases and an important
cofactor widely used by biochemical scientists. So, it was used as a pharmaceutical compound,
food additives as well as the cosmetic ingredient and elicits more interest in recent studies.
GSH is synthesized in two ATP-dependent steps (Meister and Anderso
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