浙江大学研究生免疫学英文课件抗体Ab.pptVIP

Immunoglobulin The structure and Function of Immunoglobulin (antibody) Ig genes and synthesis of protein Diversity of Ig and its mechanism Application of antibody Introduction Antibody (Ab) - protein produced by plasma cells in response to immunization and binds specifically to particular Ag. -Ab is generally defined in terms of their specific binding to the immunizing Ag Introduction Immunoglobulins (Ig) - The Igs are globulin which function as Abs or similar to Abs in chemical structure. - Igs derive their name from the finding that when Ab-containing serum is place in an electrical field, they migrate with the globular proteins - secreted Ig(sIg) ; membrane Ig(mIg) ⅠStructure and function of the Ig 1, structure 1)Heavy Chain and Light Chain heavy chain (H chain) 50~75Kd 450~550aa H chain: μ γ α ε δ isotype:IgM, IgG,IgA,IgE, IgD IgG:IgG1~4 IgA:IgA1 ,IgA2 structure of the Ig 1)Heavy Chain and Light Chain light chain (L chain) 25Kd 214aa classes of L chain: ? ? ?: ?1~ ? 4 2.variable region and constant region variable region(V region) Light Chain - VL (110 aa) Heavy Chain - VH (110 aa) HVR or CDR FR constant region(C region) Light Chain - CL (110 aa) Heavy Chain - CH (330-440 aa) : CH1, CH2, CH3,( CH4) 3. Hinge Region - between the CH1 and CH2 region - rich in proline residues flexibility: allow the two Fab arms to bind epitope; be cleaved by proteases. - IgM and IgE have no hinge region Domains of Ig Domains - globular regions of 3D images of the Ig are called domains. Light Chain Domains VL , CL 2. Heavy Chain Domains VH, CH1,CH2, CH3 (or CH4) Domains of Ig J chain and SP J chain (Joining chain) a polypeptide chain Join the monomer to polymer J chain and SP Secretory piece(SP) Ig fragmen