内质网应激在急性缺血性大鼠肾损伤中的作用-第三军医大学学报.docVIP

内质网应激在急性缺血性大鼠肾损伤中的作用 姚凤华，何娅妮，詹 俊，冀志勇 (第三军医大学大坪医院野战外科研究所肾内科，400042) 摘 要：目的研究大鼠肾脏。方法雄性SD大鼠随机分为。免疫组化检测结果1）血肌酐在缺血再灌注（I/R）后1h明显升高，12h达高峰，24h下降。肾小管损伤评分在I/R后1h升高，12h时达高峰，24h时下降。（2）肾组织中GRP78蛋白表达在I/R后6h升高达高峰，至12h仍持续较高水平，24h时下降。（3）GRP78表达与肾小管损伤呈正相关性，也与血Cr水平呈正相关性。结论 关键词：内质网应激;GRP78;肾缺血再灌注 中图法分类号：R692 文献标识码：A The potential role of endoplasmic reticulum stress in ischemia acute kidney injury of rat YAO Feng-hua, HE Ya-ni, ZHAN Jun, JI Zhi-yong (Department of Nephrology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China) Abstract：Objective　To observe the relationship between endoplasmic reticulum stress and ischemia acute kidney injury. Methods　The renal ischemia and reperfusion model was performed by suture occlusion of bilateral renal pedicles for 40min and reperfused for 1h, 6h,12h,24h. Blood SCr and BUN was detected. Renal tubular injury were measured by PAS stain. The expression of ERS marker GRP78 protein in the ischemic renal tissue was detected by immunohistochemistry. Results　The results showed that Scr increased at I/R1h, reaching a peak at I/R12h and decayed at I/R24h . The score of renal tubular injury increased at I/R1h, I/R12h group were most severely, and decayed at I/R24h. The expression of GRP78 protein in the I/R6h increased and reached the peak, then it maintained at high level in I/R12h group, and declined in I/R24h group. The expression of GRP78 protein had positive correlation with renal tubular injury, and it also had positive correlation with Scr. Conclusion　ERS was started after renal I/R in rat. It may play an important role in AKI. Key words： Endoplasmic reticulum stress; GRP78; renal ischemia / reperfusion 多种原因引起的缺血性急性肾损伤（acute kidney injury，AKI）可见于临床各科，是临床危重症之一， 基金项目:国家自然科学基金 Supported by the National Natural Science Foundation of China 作者简介:姚凤华， ， ， ， ， ， : Email: yaofenghuahh@ 通信作者:何娅妮，:(023)