杜仲MVA途径相关基因的鉴定及荧光定量PCR引物筛选精选.pdf

第33 卷 第8 期 Vol. 33 No. 8 2013 年8 月 Journal of Central South University of Forestry Technology Aug. 2013 杜仲MVA途径相关基因的鉴定及荧光定量PCR 引物筛选 12 34 12 34 （1. 中南林业科技大学 生命科学与技术学院，湖南长沙 410004 ；2. 南方林业生态应用技术国家工程实验室， 湖南长沙 410004 ；3. 中南林业科技大学 林学院，湖南长沙 410004 ； 4. 经济林育种与栽培国家林业局重点实验室，湖南长沙 410004 ） MVA KEGG MVA 30 Unigene NCBI BLAST COA , a a , 7 3 11 2 2 5 5 3 4 1 5 10 MVA SYBR GreenI PCR MVA MVA PCR S727.3 A 1673-923X(2013)08-0050-07 Related gene identifi cation of MVA pathway and primers screening of real-time PCR in Eucommia ulmoides YE Sheng-jing1,2, WUYUN Ta-na3,4, TIAN Da-lun1,2, XU Jing-shi3,4 (1. School of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; 2. National Engineering Lab. for Applied Technology of Forestry Ecology in South China, Changsha 410004, Hunan, China; 3.School of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; 4. Key Lab of Non-wood Forest Product of Forestry Ministry, Changsha 410004, Hunan, China) Abstract: Eucommia ulmoides Oliv. is a famous Chinese endemic medicinal tree being rich of terpenoids, and MVA pathway is one important way of terpenoids synthesis. From KEGG, the transcriptome data of eucommia fruit, the MVA pathway has been annotated 30 Unigene; according to BLAST comparison in NCBI, acetyl-CoA C-acetyltransfera