药学分子生物学实验指导书精选.doc

基因工程综合实验 武汉科技大学医学院生化教研室 目 录 分子生物学实验综合规程································································································3 参考书目 ··························································································································3 实验内容····························································································································4 实验一 实验基础知识和基本技能培训··········································································5 实验二 大肠杆菌感受态细胞的制备··············································································7 实验三 质粒DNA的转化·······························································································8 实验四 碱裂解法提取质粒DNA····················································································9 实验五PCR基因扩增·····································································································11 实验六DNA的限制性核酸内切酶消化········································································1实验琼脂糖凝胶电泳检测DNA·················································································15  分子生物学实验综合规程上课时间提前5分钟进入实验室；不迟到、不早退，无故旷课累计3次没成绩； 请假须医院病假条或班主任签字的假条； 上课时需穿实验服，不许在实验室内吃东西，不背着书包做实验，不穿拖鞋进实验室； 实验课期间不许打闹、闲聊等，不大声喧哗，保持课堂纪律，实验过程中，至始至终需全组同学一起完成，任何人中途不得缺席。 必须做到课前认真预习，课间做好实验记录、课后详细总结实验结果并按时提交实验报告； 公用试剂不得污染！每次都必须用新枪头。公用台上公用试剂不得私自拿到自己实验台上； 不得私自拿走或乱放别人的实验物品、实验产品； 不得擅自使用实验室中非本实验仪器、试剂。 实验废液不随便倾倒，随时保持实验台的整洁； 爱护实验器材，实验器材损坏必须按规定赔偿； 实验前按仪器性能与要求，进行仪器预热； 实验完成后检查电源插座，用完仪器后必须复位，仪器清洁干净 、摆放整齐；每组实验物品齐全，需教师验收，枪量程要归位，发现问题及时报修； 每位同学负责自己实验台面和柜体的卫生，值日生负责公用实验台、地面等处卫生； 每次轮到打扫卫生的小组，要认真做好值日工作，离开实验室须向教师声明； 同学与老师之间、同学与同学之间、实验班与实验班之间一定要互相协作配合保持公用房间卫生，注意门窗水电的安全。 遵守通用的实验室规则。 违反上述任何一条规则责任自负，并要扣分，造成损失要照价赔偿。 参考书目：分子生物学实验指导，魏群 主编，高教出版社 分子生物学实验指导，刘进元 主编，清华大学出版社 实 验 内 容 实验一 分子生物学实验的基本知识和基本技能培训 实验二 大肠杆菌感受态细胞的制备 实验三 质粒DNA的转化 实验四 碱裂解法提取质粒DNA 实验五PCR基因扩增 实验六DNA的限制性核酸内切酶消化 实验七 琼脂糖凝胶电泳检测DNA 实验一 实验基础知识和基本技能培训 实验目的 学习和掌握分子生物学实验的基本技术和技能，了解分子生物学实验中常用的仪器设备，熟悉常用玻璃器皿、耗材。 实验原理 通过多媒体、胶片、板书、实物演示等各种教学方式，让学生初步学习和掌握分子生物学实验的基