标记辅助导入中不同前景和背景选择方法的比较.pdfVIP

遗 传 学 报 Acta Genetica Sinica , December 2006, 33 (12)：1073–1080 ISSN 0379-4172 Comparison of Different Foreground and Background Selec- tion Methods in Marker-Assisted Introgression BAI Jun-Yan1,2, ZHANG Qin2,①, JIA Xiao-Ping3 1. College of Animal Science and Technology, Henan Science and Technology University, Luoyang 471003, China; 2. Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Chinese Agriculture University, Beijing 100094, China; 3. College of Biological Science, China Agricultural University, Beijing 100094, China Abstract: Three different methods for foreground selection and four different methods for background selection were compared in terms of the efficiency of marker-assisted introgression of a QTL allele from a donor line into a recipient line and also in terms of the recovery of the recipient genetic background. The results showed that for the introgression of a donor QTL allele, a direct selection on the QTL itself (when the QTL genotype can be directly identified) would ensure that the allele is successfully introgressed and rapidly fixed. However, when a direct selection on the QTL is not feasible, an indirect selection using two closely linked flanking markers can be used, which also shows similar results. For the recovery of the recipient genetic background, if the goal is to recover the whole genetic background of the recipient, genomic similarity selection or marker index selection would be the best choice: Only three generations of backcrosses were required to recover over 98% of the recipient genome. Whereas if the goal is to recover certain background traits of the recipient, MBLUP selection would give the best results, which achieved not only over 99% recovery of the recipient QTL alleles for the background