RNA interference—2001.pdfVIP

REVIEW RNA interference—2001 Phillip A. Sharp1 Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139-4307, USA In the few years since the discovery of RNA interference et al. (1999) were able to reproduce RNAi in a soluble (RNAi; Fire et al. 1998), it has become clear that this reaction. dsRNA added to this reaction is cleaved into process is ancient. RNAi, the oldest and most ubiquitous 21–23-nt RNAs, which leads to cleavage of the target antiviral system,