基因,蛋白,免疫 课程2.pptVIP

DNA sequencing Principles and Practice and the Human Genome Project. The sequencing reaction All current sequencing methods are based on that developed by Fred Sanger (MRC Lab Cambridge) Synthesis of DNA by a DNA polymerase from a single stranded template. Synthesis primed by a specific oligonucleotide. The reactions are carried out in a mixture of normal deoxynucleotides and one chain terminating di-deoxynucleotide giving rise to a population of molecules of different sizes. The synthesised strand is labelled. The population of synthesised molecules is then separated on the basis of length. S