基因,蛋白,免疫 课程之蛋白组学 5.pdf

Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator- activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription † ‡ ‡ § ¶ ‡ ‡ Juan Llopis* , Stefan Westin , Mercedes Ricote , Jiahong Wang , Charles Y. Cho* , Riki Kurokawa , Tina-Marie Mullen , David W. Rose§, Michael G. Rosenfeld§¶, Roger Y. Tsien*¶, and Christopher K. Glass‡§ Departments of *Pharmacology, ‡Cellular and Molecular Medicine, §Medicine, and ¶ Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 Contributed by Roger Y. Tsien, December 31, 1999 Members of the nuclear receptor superfamily are thought to characterized nuclear receptor coactivators at the molecular and activate transcription by recruitment of one or more recently biochemical levels. Members of this family, which include SRC-1 identified coactivator complexes. Here we demonstrate that both and p300/cAMP responsive element binding (CREB)- peroxisome proliferator-activated receptor binding protein (PBP) interacting protein (pCIP), interact with nuclear receptors Y and steroid receptor coactivator-1 (SRC-1) are required for ligand- G through a central domain that contains three copies of a O L dependent transcription of transiently t