毒理学相关资料,第五章内容参考文献19.pdfVIP

THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 274, No. 44, Issue of October 29, pp. 31185–31188, 1999 © 1999 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Mitochondrial Nitric-oxide Synthase Stimulation Causes Cytochrome c Release from Isolated Mitochondria EVIDENCE FOR INTRAMITOCHONDRIAL PEROXYNITRITE FORMATION* (Received for publication, June 2, 1999) Pedram Ghafourifar‡, Ursula Schenk, Sabine D. Klein, and Christoph Richter From the Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), CH-8092 Zurich, Switzerland Nitric oxide (NO) is synthesized by members of the NO drial membrane, its Ca2 dependence, and that the enzyme synthase (NOS) family. Recently the existence of a mito- exerts substantial control over mitochondrial respiration and chondrial NOS (mtNOS), its Ca2 dependence, and its mitochondrial transmembrane potential () (4). Soon there- relevance for mitochondrial bioenergetics was reported after the presence of mtNOS and its localization were con- (Ghafourifar, P., and Richter, C. (1997) FEBS Lett. 418, firmed, and the enzyme was enriched and shown to cross-react 291–296; Giulivi, C., Poderoso, J. J., and Boveris, A. with antibodies directed against inducible NOS (5). (1998) J. Biol. Chem. 273, 11038–11043). Here we report Apoptosis, also called programmed cell death, is an evolu- on the possible involvement of mtNOS in apoptosis. We tionary conserved phenomenon which regulates normal cellu- show that uptake of Ca2 by mitochondria