一起饮料微生物污染的鉴定报告精选.pdfVIP

Advances in Microbiology 微生物前沿, 2015, 4(4), 69-77 Published Online December 2015 in Hans. /journal/amb /10.12677/amb.2015.44010 The Identification Conclusion of a Drink Case with Microbial Contamination Limin Shi1,2, Xiaoman Li1,2, Yanhong Wang1,2, Fukai Bao1,2*, Aihua Liu2,3* 1 Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming Yunnan 2 The Institute for Tropical Medicine, Kunming Medical University, Kunming Yunnan 3 Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming Yunnan th th st Received: Dec. 10 , 2015; accepted: Dec. 28 , 2015; published: Dec. 31 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Objective: To isolate and identify the unknown microbes from the contaminated drink. Methods: With the naked eye observation of colony characteristics of isolated microorganisms inoculated in artificial culture medium, observation of colony morphology selected for staining in Medan under the microscope. The total DNA was extracted by DNA extraction kit, and used as the template, the PCR method was used to amplify the ITS gene fragment of this gene fragment, purified, sequenced by sequencing company, then the sequencing results were compared with the BLASTn tool, to find out the sequence of related strains, finally, the sequence of strains and sequence related strains in phylogenetic analysis based on the MEGA5.1 software. Result: The bacterial colony characteristics observed by naked-eye and the morphological characteristics observed microscopically show that the unknown microbe