产褐藻胶裂解酶海洋细菌的筛选和鉴定ScreeningandIdentificationof.PDFVIP

水生生物研究，2016年，第 5 期，第 10 篇，第 1-6 页 Shuisheng Shengwu Yanjiu, 2016, Vol.5, No.10, 1-6 研究报告 Research Report 产褐藻胶裂解酶海洋细菌的筛选和鉴定 1,2 2 2 2 2 2* 宋鑫 秦送玉 朱军 黄惠琴 孙前光 鲍时翔 1 海南大学环境与植物保护学院, 海口, 570228; 2 中国热带农业科学院热带生物技术研究所, 海口, 571101 * 通讯作者, baoshixiang@ 摘 要 褐藻胶是广泛存在于各种褐藻中的一类多糖物质，其降解产物褐藻寡糖具有抗肿瘤、抗凝血、抗氧 化和促进植物根部生长等作用，应用前景广阔。本研究以褐藻胶为单一碳源，从腐烂的马尾藻及其生境底泥 中共筛选获得褐藻胶降解细菌18 株。采用95%酒精处理褐藻胶裂解酶检测平板，水解圈与菌落的直径比值 (D/d) ≥5 的有13 株，D/d ≥8 的有3 株。采用DNS 法测定细菌的褐藻胶裂解酶活力，菌株DB15913 的活力 最高，达到15.42 U/mL。依据菌体形态、生理生化特征和16S rDNA 序列分析，鉴定菌株DB15913 为Cobetia amphilecti 。 关键词 马尾藻属, 褐藻胶裂解酶, 鉴定, Cobetia amphilecti, 酶活力 Screening and Identification of Marine Bacteria Producing Alginate Lyase Song Xin 1,2 Qin Songyu 2 Zhu Jun 2 Huang Huiqin 2 Sun Qianguang 2 Bao Shixiang 2* 1 College of Environment and Plant Protection, Hainan University, Haikou, 570228; 2 Institute of Tropical Bioscience and Biotechnology, Chinese A- cademy of Tropical Agricultural Sciences, Haikou, 571101 * Corresponding author, baoshixiang@ DOI: 10.13417/j.gab.035.000907 Abstract Alginate is a kind of polysaccharide which widely exists in various kinds of brown algae. Alginate oligosaccharides degraded by alginate lyase have lots of activities such as anti-tumor, anti-coagulation, anti- oxygenation and promotion for growth of plant roots. In this study, 18 bacteria were screened from rotten sargasso and its biotope using sodium alginate as the sole carbon source. By the method of 95% alcohol processing test, the result showed that the diameter ratios of hydrolyzation circle and colony (D/d) of 13 strains were larger than 5 and that of 3 strains (D/d) were larger than 8. By the method of DNS colorimetry, the fermentaion broth showed that the enzyme activity of strain DB15913 reached 15.42 U/mL. According to morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis, strain DB15913 was