生防链霉菌SAT1的分离鉴定及其对辣椒疫霉的生物-广东农业科学.PDFVIP

广东农业科学 2014 年第 1怨 期 75 生防链霉菌SAT1 的分离、鉴定及其 对辣椒疫霉的生物防治潜力 1 2 1 刘晓瑜 窦桂铭 马玉超 1. 北京林业大学生物科学与技术学院袁 北京 100083 曰 2.北京林业大学林学院袁 北京 100083 冤 要 椒疫霉引起的植物病害使农业遭受了巨大的经济损失袁环保尧经济实用的混合微生物菌剂防治策略 是当前的最新发展趋势遥 试验从 6 种药用植物分离得到 15 株植物内生菌袁 平板拮抗实验筛选得到拮抗链霉菌 SAT1 袁经鉴定为Stre ptomyc es anandii 袁其发酵液粗提物和挥发性物质对 椒疫霉菌丝生长有明显抑制作用遥接种链霉 菌 SAT1 的孢子液可提高 椒苗对 椒疫霉的抗性并明显促进 椒苗的生长遥 关键词 椒疫霉曰 植物内生菌曰 生物防治 + 中图分类号 S482.2 92 文献标识码 A 文章编号 1004-874X 2014冤19-0075-05 Screening and identification of antagonistic SAT1 and its potential for biocontrol of pepper phytophthora blight 1 2 1 LIU Xiao-yu , DOU Gui-ming , MA Yu-chao (1.College of Biological Sc ie nce s and Biotec hnology , Be ij ing Fores try Unive rs ity , Be ij ing 100083, China; 2.Colle ge of Fore s try, Be ij ing Fores try Univ e rs ity , Be ij ing 100083, China) Abstract: Numerous soft rots of pepper are caused by Phytop hthora caps ici, w hich give rise to serious economic losses of agriculture. A n emerging trend is apply ing a mixture of diverse group of microorganisms as the most economically feasible practical strategy . A total of 15 endophytes were isolated from 6 medicinal plants, and an antagonistic Strep tomyces SAT1 were screened by antagonism assays and was identified as S. anandii, the crude extract from the fermented liquid and volatile substances of SAT 1 obviously inhibited the growth of Phytop