基于2b-RAD简化基因组测序的半滑舌鳎群体 遗传多样性分析.pdfVIP

Open Journal of Fisheries Research 水产研究, 2017, 4(4), 125-133 Published Online December 2017 in Hans. /journal/ojfr /10.12677/ojfr.2017.44020 Analysis of Population Genetic Diversity of Cynoglossus cynoglossus Based on 2b-RAD Simplified Genome Sequencing * Lei Jia, Bo Zhang , Kefeng Liu, Debin Zheng, Xiaoyu Wang Tianjin Bohai Sea Fisheries Research Institute, Bohai Sea Fisheries Research Center of Chinese Academy of Fishery Sciences, Tianjin st rd th Received: Oct. 21 , 2017; accepted: Nov. 3 , 2017; published: Nov. 16 , 2017 Abstract Taking the cultured and wild populations of Cynoglossus semilaevis in Tianjin coast and cultured populations in Haiyang as study objects, there were 32,746 SNP locis screened by 2b-RAD se- quencing. The genetic relationship, genetic structure, genetic diversity in these three groups were also analyzed by SNP markers. The genetic variation within populations (Fis), the genetic varia- tion between groups (Fst), and total variation (Fit) in each SNP locus in these three groups were estimated, which shown that Fst value was ranged from 0.0731 - 0.1635. The genetic differentia- tion between Tianjin culture population and Tianjin wild population is the smallest, and the ge- netic differentiation between Tianjin and Haiyang culture populations, Tianjin wild population and Haiyang culture population is great. The results of selective sweep showed that the polymor- phism of wild population was highest in Tianjin, followed by Tianjin culture population, and low- est in Haiyang breeding population. The polymorphism information content (PIC) statistics of Haiyang, Tianjin wild and