酶法制备磷酸葡萄糖合成条件的响应曲面法优化.PDFVIP

王晓娟 等/酶法制备 1-磷酸葡萄糖合成条件的响应曲面法优化 107 生 物 工 程 学 报 Chinese Journal of Biotechnology January 25, 2013, 29(1): 107−110 /cjbcn ©2013 Chin J Biotech, All rights reserved 生物育种与工艺优化 酶法制备 1-磷酸葡萄糖合成条件的响应曲面法优化 王晓娟，靳利娥，畅芬芬，阎果兰 太原理工大学化学化工学院，山西 太原 030024 王晓娟, 靳利娥, 畅芬芬, 等. 酶法制备 1-磷酸葡萄糖合成条件的响应曲面法优化. 生物工程学报, 2013, 29(1): 107−110. Wang XJ, Jin LE, Chang FF, et al. Optimization of enzymatic preparation of glucose 1-phosphate by response surface methodology. Chin J Biotech, 2013, 29(1): 107−110. 摘 要: 以葡萄糖为原料，三聚磷酸钠为磷酰化试剂，马铃薯磷酸化酶为催化剂，制备 1-磷酸葡萄糖。利用 Box-Behnken 实验设计原理，采用三因素三水平的响应面分析法，以产物含量为响应值考察温度、物料比 ( 葡萄 糖/三聚磷酸钠) 、时间3 个因素影响。采用氢核磁共振波谱仪对产品进行了分析。结果表明，马铃薯磷酸化酶制 备葡萄糖磷酸酯的最佳工艺条件为：温度35 ℃，葡萄糖与三聚磷酸钠物料比为1.35 ∶1 (mol/mol)，时间19 h。 关键词: 马铃薯磷酸化酶，1-磷酸葡萄糖，响应曲面法 Optimization of enzymatic preparation of glucose 1-phosphate by response surface methodology Xiaojuan Wang, Li’e Jin, Fenfen Chang, and Guolan Yan College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China Abstract: With glucose as substrate, sodium tripolyphosphate as the phosphorus acylating agent, and phosphorylase of Solanum tuberosum as the catalyst, glucose 1-phosphate was synthesized. Based on a three-level, three-variable Box-Behnken experimental design, response surface methodology was used to evaluate the effects of temperature, molar ratio of glucose to sodium tripolyphosphate and time on the production. The structure of the product was confirmed by 1H NMR spectra. The results show that the optimum conditions were as follows: temperature 35 °C, molar ratio of glucose to sodium tripolyphosphate 1.35:1 and time 19 h. Keywords: