心力衰竭--糖尿病患者急性心肌梗死后无法回避的难题PPT.pptVIP

心力衰竭 --糖尿病患者急性心肌梗死后无法回避的难题;糖尿病患者急性心肌梗死后心力衰竭;;;;Diabetes and congestive HF independent of CAD;糖尿病急性心肌梗死后晚期HF的机制;Diabetes mellitus can accerate the progression of post-infarction genetic regulatory expression in untreated Streptozotocin-induced Diabetic Rat Model -Genetic findings in the remote zone of LV free wall post acute myocardial infarction Guang-Yuan Song1, Yong-Jian Wu1*, Yue-Jin Yang1, Jian-Jun Li1, Rui Li2, Ru-Tai Hui3, Han-Jun Pei1, Zhen-Yan Zhao1 From the 1Center of Coronary Heart Disease, 3Center of Hypertension, Cardiovascular Institute Fu-Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences , Beijing 100037 , China. From the 2Genminix Informatics Ltd.Co *The corresponding author: Yong-Jian Wu, MD, PhD; Center of Coronary Heart Disease, Department of Cardiology, Cardiovascular Institute Fu-Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 167 BeiLiShi Rd, Beijing, 100037, P.R. China. E-mail: fuwaihospital@;Study Design 217 Sprague-Dawley (SD) rats were randomized into one of the four following groups: (1) AMI in diabetic rats (DM + AMI); (2) AMI in non-diabetic rats (N-DM + AMI); (3) Sham in diabetic rats (DM + Sham);(4) Sham in non-diabetic rats (N-DM + Sham). Experimental protocol is shown in Figure 1;Aminals were sacrificed just after echocardiographic assessment, and the remote zone tissues of LV free wall were taken as samples at day 1, 7, 14, 28, and 56 post AMI. According to previous studies , we used the sample pooling strategies for microarray analysis in order to reduce the whole cost of the study. RNA fractions from the three rats in each group at the time point were balanced pooled for GeneChip analysis. Significant different expression genes were filtered from Affymetrix Genechip U230 2.0 array by GCOS software (P0.01). Genetic changes post myocardial infarction were classified by hierarchical clustering. And then, the differential expressions of 10 selected transcr