烟草基因瞬时表达体系的建立与优化研究精选.pdfVIP

Botanical Research 植物学研究, 2015, 4, 25-31 Published Online March 2015 in Hans. /journal/br /10.12677/br.2015.42004 Establishment and Optimization of Agrobacterium-Mediated Transient Gene Expression System in Tabacco Liwei Wen, Hongliang Zhu* Department of Food Science and Nutritional Engineering, China Agricultural University, Beijing * Email: 210824@, hlzhu@ th st th Received: Apr. 15 , 2015; accepted: May 1 , 2015; published: May 6 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract To establish and optimize a transient expression system in Nicotiana benthamiana, the method was developed with the β-glucuronidase (GUS) and Ripening Inhibitor (RIN) as marker genes. Us- ing the agrobacterium-mediated transformation method, GV3101 was used for the effects of dif- ferent bacteria concentration on the efficiency of protein transient expression in Nicotiana ben- thamiana. Observed by the results, a higher transient expression level of GUS RIN gene could be obtained as OD600 value of A. tumefaciens for intiltration 1.0. The entire process only took 25 days from sowing seed to protein analysis. Therefore, this method is simple and rapid. It has a potential application in dissecting gene expression and function in Brassica napus. Keywords Tobacco, Leaf, Transient Expression System 烟草基因瞬时表达体系的建立与优化研究 文莉薇，朱鸿亮* 中国农业大学，食品科学与营养工程系，北京 * Email: 210824@, hlzhu@ *通讯作者。 25 烟草基因瞬时表达体系的建立与优化研究