超顺磁性壳聚糖质粒(pDsVEGF165Red1-N1)明胶控释微球修复颅骨缺损的实验研究.docVIP

精品论文 参考文献 超顺磁性壳聚糖质粒(pDsVEGF165Red1-N1)明胶控释微球修复颅骨缺损的实验研究 周建军，梁平 ，罗聪 　　(重庆医科大学附属儿童医院神经外科 重庆市，400014) 　　摘要 目的 通过观察超顺磁性壳聚糖质粒(pDsVEGF165Red1-N1)明胶微球(SPCPGM)与磷酸钙骨水泥的复合支架在外加振荡磁场下修复颅骨缺损的作用，探讨其成骨效果。方法 将50只新西兰大白兔随机分为5组，并制成颅内颅骨缺损模型，分别植入超顺磁性壳聚糖质粒明胶微球(SPCPGM- VEGF165)/多孔磷酸钙骨水泥(CPC)复合支架、超顺磁性壳聚糖明胶微球((SPCPG)/多孔磷酸钙骨水泥(CPC)复合支架、多孔磷酸妈骨水泥(CPC)支架，经振荡磁场处理。于术后2周、4周、8周、12周时通过大体、X线检测、组织切片观察血管化及成骨情况，图像分析系统分析、求积仪测量对骨缺损处成骨情况进行测定，评估各组成骨的情况。结果 在振荡磁场下超顺磁性壳聚糖质粒明胶微球(SPCPGM- VEGF165)/多孔磷酸钙骨水泥(CPC)复合支架组支架开始降解吸收速度、血管化、成骨作用优于对照组。结论 在振荡磁场下超顺磁性壳聚糖质粒明胶控释微球局部控释增加了持续作用时间，同时促进了质粒VEGF165细胞转染，从而促进新生骨血管化及成骨的作用，可用于颅骨缺损修复。 　　关键词: 颅骨缺损;超顺磁性纳米微球;振荡磁场;质粒VEGF165 　　中图分类号R944 文献标识码A 　　The study of superparamagnetic chitosan (pDsVEGF165)gelatin 　　microsoheres for the cranial defects 　　Zhou Jian-jun, Linag ping, Luo cong 　　(Department of Neurosurgery, the Childrens Hospital, Chongqing Medical University, Chongqing ,400014) 　　Abstact Objective: To observe the advance of cranial bone defects healing of the porosity scaffolds filled with the SPCPGM thin the oscillating magnetic feild, to investigate the effect of osteogenesis and clinical application value. Methods: Product sufficent recombinant plasmids vascular endothelial growth factor (VEGF165 )that can expresse in the demic cells; The superparamagnetic chitosan gelatin microspheres(SPCGM)were prepared by the chemical co-precipitation; The super paramagnetic chitosan plasmid(pDsVEGF165)compounds(SPCPGM) were prepared with combination; The SPCPGM and SPCGM were all prepared by the method of emulsification cross-linked. The porosity scaffolds were made of the cement of the calcium phosphate ceramic(CPC)and gelatin microspheres composite; Then the specimens were obtained in 2, 4, 8 and 12 weeks postoperatively. Observatiived the ability of cranial bone skull defects healing in different groups. Result: The study indicates that SPCPGM promote the osteogenesis in cranial bone defects healing by oscillating magnetic field. Group E are better than others. The or