清醒家兔心绞痛模型的建立.pdfVIP

- 34 - Drug Evaluation Research, Vol.32 No.1, August 2009 清醒家兔心绞痛模型的建立 徐 旭，席文恭，赵专友，汤立达 天津药物研究院药代与药效动力学重点实验室，天津 300193 摘 要 目的：制备清醒家兔心绞痛模型，以便更好的进行抗心绞痛药物研究。方法：家兔在固定盒中适应性训练一段时间 后，右前肢及双后肢电极放置区去毛，贴附带泡沫垫的 Ag/AgCl 电极，连接多导生理记录仪测定 Ⅱ 导联心电图。 耳缘 iv 血管加压素 0.05 IU/kg 诱发心绞痛。并观察硝苯地平、硝酸甘油对该模型的影响。结果：清醒家兔 iv 加 压素 0.05 IU/kg ，可导致心电图 T 波电压升高。给予硝苯地平、硝酸甘油可减弱加压素引起的 T 波抬高。结论： 该模型结果可靠，有利于抗心绞痛药物的研究。 关键词 心绞痛；动物模型；清醒动物；硝苯地平；硝酸甘油 中图分类号：R965.1 文献标志码：A 文章编号：1674–6376 （2009 ）01–0034–04 Establishment of awake rabbits model suffered from angina pectoris XU Xu, XI Wen-gong, ZHAO Zhuan-you, TANG Li-da Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China Abstract Objective: To establish the awake rabbits model suffered from angina pectoris in order to carry out the study on anti-angina drug better. Methods: Rabbits were fixed for the adaptive training in boxes for a period of time. The Ag/AgCl disposable electrodes with foam pad were attached to the right forelimb and hind pairs of rabbits with hair cut to determine ECG Ⅱ. The angina pectoris was induced by iv injection of Vasopressin 0.05 IU/kg to rabbit ear and the effect of Nifedipine or Nitroglycerin on the model suffered from angina pectoris was observed. Results: After iv injection of vasopressin 0.05 IU/kg, ECG T-wave voltage of the awake rabbits was increased. Nifedipine or Nitroglycerin could reduce T-wave elevation caused by Vasopressin. Conclusion: The awake rabbits model suffered from angina pectoris is reliable an