不同压力高压氧连续治疗对永久性MCAO大鼠细胞增殖的干预作用.DOC

不同压力高压氧连续治疗对永久性MCAO大鼠细胞增殖的干预作用 曾令琼1 , 曾晓鹏1，牟君1 ,杨泽松(2 （4000161重庆，1：重庆医科大学附属第一医院神经内科； 2．重庆医科大学附属第一医院血液内科） [摘要] 目的：比较延迟、连续给予不同压力（1.5 ATA和2.5 ATA）的高压氧治疗（Hyperbaric Oxygen Therapy, HBOT），对永久性大脑中动脉闭塞（Permanent middle cerebral artery occlusion, pMCAo)大鼠神经发生的影响。方法：雄性SD 大鼠80 只，分为假手术组，pMCAo +空气组，pMCAo +HBOT（1.5 ATA）组和pMCAo + HBOT（2.5 ATA）组，每组20只。采用大脑中动脉线栓法建立永久性大鼠缺血模型。于术后48小时开始高压氧治疗，连续10天，每天1小时。于术后28天评估神经功能、脑梗死面积和神经发生情况。结果： 2.5 ATA HBOT组与1.5 ATA HBOT组及空气组比较能有效减少死亡率和梗死面积，增加梗死周边区域的神经发生，并上调该区域CREB的表达（P0.05）；而1.5 ATA HBOT组与空气组比较，上述指标无明显差异（P0.05）。结论：2.5 ATA高压氧的神经保护作用可能与CREB上调促进梗死后神经发生有关。 [关键词] 高压氧；永久性大脑中动脉闭塞；神经发生 [中图分类号]：R743 文献标识码：A Effect of hyperbaric oxygen therapy on neurogenesis in permanent MCAo rats Zeng Lingqiong1, Zeng Xiaopeng1, Mu Jun1, Yang Zesong*2 (1.Department of Neurology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016; 2 .Department of Hematology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016) [Abstract]: Objective To explore the optimal pressure(1.5 ATA and 2.5 ATA) of Hyperbaric Oxygen Therapy(HBOT) to promote neurogenesis in the permanent middle cerebral artery occlusion(pMCAo) rats. Methods Male SD rats were randomly divided into 4 groups, including sham+air,pMCAo +air,pMCAo +HBOT（1.5 ATA）and pMCAo + HBOT（2.5 ATA）(n=20). HBOT (100% O2) was started 48 hrs after pMCAo, 1 hr daily over 10 consecutive days. Garcia Score and beam balance test were performed 28 days after pMCAo to evaluate neurological deficits.The infarct size and neurogenesis were analyzed by immunohistochemistry. Western blotting was used to detect the level of cAMP response element-binding protein (CREB).Results Compared to the air group, 2.5 ATA HBOT group effectively reduced the mortality and infarct size. Also it increased the peri-infarct region neurogenesis with up-regulated expression of CREB. Conclusion 2.5 ATA HBTO might promote the neurogenesis after pMCAo through CREB expression. Key Words: Hyperbaric oxygen therapy; pM