甘草甜素对大鼠肾小球硬化早期的防护作用.pdfVIP

50 中草药 Chinese Traditional and Herbal Drugs 41 010 * 于 力,张 蕾,郝志宏, 王丽娜, 邓 颖,张又祥 ( , 510180) : iv 3 ,468 ,24 h ( BUN) ( SCr) (T C) (Alb) 1 ( TGF1) ( CTGF) PCR TGF1CTGF 1 ( TI P1) mRNA 24 h BUNSCrTC Alb (P 005) TGF1 CTGF , (P 005) TGF1 CT GF T I P1 mRNA ,(P 005) mRNA TGF1CTGF TI P1, :; ; 1( TGF1) ; ( CT GF); 1 ( TI P1) :R2855 :A : 0253 2670(2010) 02 0250 06 Renoprotective effects of glycyrrhizin on experimental glomerulosclerosis in rats YU Li, ZHANG Lei, HAO Zhihong, WANG Lina, DENG Ying, ZHANG Youxiang ( Department of Pediatrics, Guangzhou First unicipal Peoples Hospital. Guangzhou 510180, China) Abstract: Objective To investigatethe protective effects of glycyrrhizin (GL) on early glomeruloscle rosis. Methods To create glomerulosclerosis models of rat by injecting adriamycin twice in vena caudalis. The rats were randomly divided into following three groups: model group, treatment group, and control group. The level of each index was measured at theweek4, 6, and 8. Theindexes include: quantitation of 24 h urine protein, serum levels of blood urea nitrogen ( BUN), serum creatinine ( SCr) , cholesterol (TC), albumin ( Alb) ; Histopathologic examination for renal cortex of each group, including light micro scope was carried out; Detection of the expression for transforming growth factor1 ( TGF1), connec tive tissue growth factor (CTGF) in the renal cortex by the immunohistochemical method; Detection of the expressione of mRNA of TGF1, CTGF, and tissue inhibitor of metalloproteinase1( TI P1) by stand ard curve with fluorescent quantitation PCR were conducted. Results Treatment group showed an im provement in quantitation of 24 h urineprotein, BUN, SCr, Alb, and TC compared with the model grou