Relation of Polyamine Synthesis and Titer to Aging and英文资料.pdfVIP

Plant Physiol. (1982) 69, 405-410 0032-0889/82/69/0405/06/$00.50/0 Relation of Polyamine Synthesis and Titer to Aging and Senescence in Oat Leaves Received for publication May 14, 1981 and in revised form September 28, 1981 RAVINDAR KAUR-SAWHNEY, LIu-MEI SHIH, HECTOR E. FLORES, AND ARTHUR W. GALSTON Department ofBiology, Yale University, New Haven, Connecticutt 06511 ABSTRACT plants, the ADC pathway semes to be preferred in some cases (21, 27), while in animal tissues the ODC pathway predominates (4, Polyamine biosynthesis in senescing leaves of Avena sativa L. was 5). The Put formed by either pathway is converted to Spd and measured by determining the activities of arginine decarboxylase (EC Spm by the consecutive addition of propylamino residues origi- 9), ornithine decarboxylase (EC7) and S-adenosyl-L-methio- nating from decarboxylated SAM generated via SAMDC (4, 5, nine decarboxylase (EC0). Polyamine content was also estimated 27). by thin layer chromatography and high performance liquid chromatography. In plants, high levels of ADC activity have been reported in Arginine decarboxylase activity decreases progressively in aging attached growing seedlings of Lathyrus sativus (22), during embryogenesis first leaves and in senescing excised leaves in the dark. Conversely, it of carrot cell suspension cultures (19), and in tissues subjected to increases during Ught exposure ofexcised leaves, which retards senescence. light and growth stimuli, high salinity, or potassium deprivation Ornithine decarboxylase activity is high and constant in the attached leaf, (6, 18, 27, 29). Increased ODC activity has been reported in irrespective of age; it decreases in excised leaves kept in