ageing and selenite induced cataractogenesis in Wistar rats英文资料.pdfVIP

Molecular Vision 2010; 16:445-453 /molvis/v16/a50 © 2010 Molecular Vision Received 14 February 2010 | Accepted 10 March 2010 | Published 16 March 2010 Alterations in lenticular proteins during ageing and selenite- induced cataractogenesis in Wistar rats 1 1 2 1 Muniyan Sakthivel, Rajan Elanchezhian, Philip A. Thomas, Pitchairaj Geraldine 1 2 Department of Animal Science, School of Life Sciences, Bharathidasan University, Tamil Nadu, India; Institute of Ophthalmology, Joseph Eye Hospital, Tamil Nadu, India Purpose: To determine putative alterations in the major lenticular proteins in Wistar rats of different ages and to compare these alterations with those occurring in rats with selenite-induced cataract. Methods: Lenticular transparency was determined by morphological examination using slit-lamp biomicroscopy. Alterations in lenticular protein were determined by sodium dodecyl sulfate(SDS–PAGE) and confirmed immunologically by western blot. Results: Morphological examination did not reveal observable opacities in the lenses of the rats of different age groups; however, dense nuclear opacities were noted in lenses of rats in the selenite-cataract group. Western blot assays revealed age-related changes in soluble and urea-soluble lenticular proteins. Decreased αA- and βB1-crystallins in the soluble fraction and aggregation of αA-crystallin, in addition to the degraded fragment of βB1-crystallin, in the urea-soluble fraction appeared to occur in relation to increasing age of the rats from which the lens