B4 Project Simple Sequence Repeat (SSR) Analysis英文资料.pdfVIP

B4 Project Simple Sequence Repeat (SSR) Analysis Theo R. Allnutt, Chris Conyers. Fera Sand Hutton YO411LZ Results Summary DNA was extracted from12 worker bees, sampled from eight colonies provided by the B4 project. PCR amplification was successful for all samples and all 12 SSR loci. The mean number of alleles (a relative estimate of genetic diversity) in each colony were: #1 = 3.58; #2 = 3.5; #3 = 3.42; #4 = 3.64; #5 = 3.25; #6 = 2.67; #7 = 4.42; #8 = 3.50. 1. Principal Coordinates Analysis (PCO) A pairwise distance measure was calculated for all B4 bees based on the proportion of shared SSR alleles. PCO was performed using Genstat 15.3. The first two principal coordinates contained 36% and 10.5% of the total variation (these are relatively high values, indicating the is a high degree of structure, e.g. clusters in the data). Figure 1 shows the genetic separation between the Fera reference queen bee samples and the B4 samples. Although B4 samples partially overlap with the UK Apis melifera melifera (AMM) samples, they are also distinct from them. Figure 1. PCO plot. Numbers 1- 8 on the PCO plot represent B4 colonies 1 to 8. ‘C’ and ‘N’ represent Colonsay and Northumbria components of the UK Apis melifera melifera (AMM) reference population. HI-CAR = Hawaian carnica; SI-CAR = Slovenian carnica; DE-CAR = German carnica; HI-LIG = Hawaian ligustica; NZ-LIG = New Zealand ligustica; AUS-LIG = Australian ligustica; Balkan = balkan / greek sub species; UK melifera = A. melifera melifera ; FR-HYB = French AMM hybrids; ES-IB = Spanish A. m. iberica . 2. Bayesian Analysis of Populations (BAPs) BAPs 5. software (http://www.helsinki.fi/bsg/software/BAPS/), was used in ‘assignment to predefined populations’ mode, which calculated a probability that each individual’s allele